Plant Physiol.
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Plant Physiology 49:482-489 (1972)
© 1972 American Society of Plant Biologists

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The Influence of Axis Removal on Protein Metabolism in Cotyledons of Pisum sativum L. 1

T. Y. Chin2, Rozanne Poulson3 and Leonard Beevers4

a Department of Horticulture, University of Illinois, Urbana, Illinois 61801

The protein metabolism of cotyledons attached to the embryonic axis has been compared with that in cotyledons removed from the axis at the initiation of a 6-day imbibition. Total protein declined in the attached but not in the detached cotyledons. Concurrent with the decline in protein level in the intact cotyledons there was an increased capacity to incorporate exogenously supplied leucine into protein. In contrast, detached cotyledons showed a restricted capacity for protein synthesis. It was demonstrated that ribosomal preparations from cotyledons of intact seedlings contained an increasing proportion of polyribosomes as germination progressed and such ribosomes were active in in vitro amino acid incorporation. Ribosomal preparations from detached cotyledons contained few polyribosomes and had a restricted capacity to incorporate amino acids in vitro. The in vitro incorporation of phenylalanine was stimulated by polyuridylic acid with the stimulation being greatest in ribosomal preparations from detached cotyledons. The results suggest that an axis component may regulate the availability of messenger RNA in the cotyledons during germination.

2 Present address: Department of Biology, Nanyang University, Singapore.

3 Present address: Department of Biochemistry, University of British Columbia, Vancouver 8, British Columbia, Canada.

4 Present address, to which reprint requests should be mailed: Department of Botany and Microbiology, University of Oklahoma, Norman, Okla. 73069.

1 Research supported by Grant GB 15189 from the National Science Foundation.






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Copyright © 1972 by the American Society of Plant Biologists