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Enhanced Deoxyribonucleic Acid Polymerase Activity of Chromatin from Soybean Hypocotyls Treated with 2,4-Dichlorophenoxyacetic Acid ¹

^a Departments of Agronomy and Horticulture, Purdue University, Lafayette, Indiana 47907

Chromatin isolated from soybean (Glycine max L., var. Wayne) hypocotyls was capable of catalyzing the polymerization of labeled deoxyribonucleoside triphosphate in the presence of the three other deoxyribonucleoside triphosphates into a trichloroacetic acid-insoluble product. This product was insensitive to base hydrolysis and ribonuclease, but was sensitive to acid hydrolysis and deoxyribonuclease. Chromatin-DNA polymerase required Mg²⁺ and all four deoxyribonucleoside triphosphates for maximal activity. Inorganic pyrophosphate and actinomycin D inhibited the polymerase activity, but 2, 4-dichlorophenoxyacetic acid had no effect in vitro. Chromatin from plants previously treated with 2, 4-dichlorophenoxyacetic acid supported a greater level of DNA synthesis than did chromatin from untreated plants.

³ Present address: Allan Hancock Foundation, Department of Biological Sciences, University of Southern California, Los Angeles, Calif. 90007.

¹ Purdue University Agriculture Experiment Station Journal Paper 4304. Taken in part from a dissertation submitted by the senior author to the Graduate School of Purdue University in partial fulfillment of the requirements for the Ph.D. degree.