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Plant Physiology 47:400-403 (1971)
© 1971 American Society of Plant Biologists

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Kinetin Inhibition of 3H-Uracil and 14C-Leucine Incorporation by Tobacco Cells in Suspension Culture

Uri Nudel and Elchanan S. Bamberger

Department of Biochemistry, Tel-Aviv University, Tel-Aviv, Israel

The rate of 14C-leucine and 3H-uracil incorporation by tobacco cells (Nicotiana tabaccum var. Samsun N.N.) in suspension culture was simultaneously decreased by the addition of kinetin at concentrations above 2.5 x 10–5M. Ribosomal RNA was the first RNA species affected by kinetin. The purine derivatives, adenine and N6-methyl-aminopurine, which exhibit low cytokinin activity overcame the inhibitory effects of kinetin. However, purine derivatives without cytokinin activity, guanine, N6,6-dimethyl-aminopurine, and 2-aminopurine, did not relieve kinetin inhibition.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1971 by the American Society of Plant Biologists