Plant Physiol. Bio-Rad Microplate Reader
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 47:342-345 (1971)
© 1971 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spiker, S.
Right arrow Articles by Chalkley, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spiker, S.
Right arrow Articles by Chalkley, R.
Agricola
Right arrow Articles by Spiker, S.
Right arrow Articles by Chalkley, R.
Articles

Electrophoretic Analysis of Histones from Gibberellic Acid-treated Dwarf Peas

Steven Spikera,1

Roger Chalkleyb

a Department of Botany, University of Iowa, Iowa City, Iowa 52240, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52240

Histones from the epicotyls of light-grown dwarf peas (Pisum sativum L. cv. Little Marvel) which had been treated with gibberellic acid were compared to histones from control dwarf peas by the method of polyacrylamide gel electrophoresis. The histone complements were found to be unaltered in the electrophoretic mobility and relative quantity of the individual fractions. The ratio of histone to DNA was also unaffected by treatment with gibberellic acid. The investigation confirmed earlier reports that over 95% of the histone of peas is contained in seven molecular species and that one of these can exist both as an oxidized disulfide dimer and as a reduced monomer. Evidence is presented which indicates that only the monomer form exists in vivo in the pea epicotyl tissue and that the oxidized dimer is an artifact of extraction. The implications of the data concerning the mechanism of action of gibberellic acid are discussed.

1 Present address: Division of Biology, Kansas State University, Manhattan, Kansas 66502.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1971 by the American Society of Plant Biologists