This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nevins, D. J. Search for Related Content PubMed PubMed Citation Articles by Nevins, D. J. Agricola Articles by Nevins, D. J.

Articles

Relation of Glycosidases to Bean Hypocotyl Growth ¹

^a Department of Botany and Plant Pathology, Iowa State University, Ames, Iowa 50010

The enzymes -glucosidase, -glucosidase, -galactosidase, -galactosidase, and -xylosidase were detected in Phaseolus vulgaris L. var. Red Kidney bean hypocotyl tissue throughout the first 13 days of development with p-nitrophenyl glycosides as substrates. Activities of all enzymes except -glucosidase declined as a function of increasing tissue age. In contrast, -glucosidase activity increased rapidly 3 days after imbibition to a maximal activity at 5 days and then subsided to one-third the maximum by day 7. This activity peak immediately preceded the logarithmic phase of hypocotyl growth. This enzyme is strongly associated with cell walls during extraction, suggesting that it is wall-bound in situ. Various polysaccharide substrates were used to evaluate the specificity of this enzyme.