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Hormonal Control of Cell Proliferation and Xylem Differentiation in Cultured Tissues of Glycine max var. Biloxi ¹

^a The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138

The relationship between tracheary element differentiation, cell proliferation and growth hormones was examined in agar-grown soybean callus. The time course of cell division and tracheary element formation in tissues grown on a medium containing 5 x 10^–7M kinetin and 10^–5M NAA was determined by means of maceration technique. After a slight lag period, a logarithmic increase in cell number was observed through the twelfth day of the culture period. Cell numbers increased at a considerably slower rate after the twelfth day. The rate of tracheary element formation varied with the rate of cell proliferation. Tracheary elements increased logarithmically during the log phase of growth. As the rate of cell division decreased after the twelfth day of culture, the rate of tracheary element formation also decreased. In the presence of 10^–5M NAA, cell number increased as the kinetin concentration was increased between 10^–9 and 10^–6M. However, tracheary element formation was not initiated unless the kinetin concentration was 5 x 10^–8M or above. When the Biloxi callus was subcultured repeatedly on media containing 10^–8M kinetin, a tracheary element-free population of cells was obtained. This undifferentiated tissue produced tracheary elements upon transfer to a medium containing 5 x 10^–7M kinetin. In the presence of 5 x 10^–7M kinetin, NAA stimulated cell proliferation between 10^–7 and 10^–5M, but no tracheary elements were formed without auxin, or with 10^–7M NAA. Neither NAA nor kinetin at any concentration tested stimulated tracheary element formation in the absence of an effective level of the other hormone. However, 2,4-D at 10^–7 or 10^–6M promoted both cell proliferation and tracheary element differentiation in the absence of an exogenous cytokinin.

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