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Plant Physiology 43:1769-1780 (1968) © 1968 American Society of Plant Biologists Developmental Physiology of Bean Leaf Plastids II. Negative Contrast Electron Microscopy of Tubular Membranes in Prolamellar Bodies 1Department of Biological Sciences, Purdue University, Lafayette, Indiana 47907
Proplastids and prolamellar bodies with tubular membranes were isolated from the dark grown primary leaves of bean seedlings (Phaseolus vulgaris L.). The combination of fluorescence microscopy and negative contrast electron microscopy provided the tentative identification of protochlorophyll holochrome as a constituent of prolamellar body membranes and new evidence for solution-filled channels within the tubular membrane systems of prolamellar bodies.
1 This investigation was supported by NSF grant GB-2897.
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