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Plant Physiology 141:1185-1193 (2006)
© 2006 American Society of Plant Biologists

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BREAKTHROUGH TECHNOLOGIES

Cosecretion of Protease Inhibitor Stabilizes Antibodies Produced by Plant Roots1

Slavko Komarnytsky*, Nikolai Borisjuk2, Nir Yakoby3, Alison Garvey and Ilya Raskin

Biotech Center, Rutgers University, New Brunswick, New Jersey 08901

A plant-based system for continuous production of monoclonal antibodies based on the secretion of immunoglobulin complexes from plant roots into a hydroponic medium (rhizosecretion) was engineered to produce high levels of single-chain and full-size immunoglobulins. Replacing the original signal peptides of monoclonal antibodies with a plant-derived calreticulin signal increased the levels of antibody yield 2-fold. Cosecretion of Bowman-Birk Ser protease inhibitor reduced degradation of the immunoglobulin complexes in the default secretion pathway and further increased antibody production to 36.4 µg/g root dry weight per day for single-chain IgG1 and 21.8 µg/g root dry weight per day for full-size IgG4 antibodies. These results suggest that constitutive cosecretion of a protease inhibitor combined with the use of the plant signal peptide and the antibiotic marker-free transformation system offers a novel strategy to achieve high yields of complex therapeutic proteins secreted from plant roots.

1 This work was supported in part by grants from Phytomedics, Inc. (Dayton, NJ), Rutgers, the State University of New Jersey, and N.J. Agricultural Experiment Station, and in part by Vaadia-BARD (postdoctoral award no. FI–302–2000 to N.Y.).

2 Present address: Thomas Jefferson University, 1020 Locust St., Philadelphia, PA 19107.

3 Present address: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Slavko Komarnytsky (komar{at}aesop.rutgers.edu).

www.plantphysiol.org/cgi/doi/10.1104/pp.105.074419.

* Corresponding author; e-mail komar{at}aesop.rutgers.edu; fax 732–932–6535.

Received November 23, 2005; returned for revision April 14, 2006; accepted May 18, 2006.

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