This Article Full Text Full Text (PDF) All Versions of this Article: 139/2/991    most recent pp.105.066407v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Xu, M.-J. Articles by Zhu, M.-Y. Search for Related Content PubMed PubMed Citation Articles by Xu, M.-J. Articles by Zhu, M.-Y. Agricola Articles by Xu, M.-J. Articles by Zhu, M.-Y. Related Collections Reactive Oxygen Species

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Nitric Oxide Mediates the Fungal Elicitor-Induced Hypericin Production of Hypericum perforatum Cell Suspension Cultures through a Jasmonic-Acid-Dependent Signal Pathway¹

State Key Lab of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China (M.-J.X., M.-Y.Z.); and Department of Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, People's Republic of China (M.-J.X., J.-F.D.)

Fungal elicitor prepared from the cell walls of Aspergillum niger induces multiple responses of Hypericum perforatum cells, including nitric oxide (NO) generation, jasmonic acid (JA) biosynthesis, and hypericin production. To determine the role of NO and JA in elicitor-induced hypericin production, we study the effects of NO scavenger 2- to 4-carboxyphenyl-4,4, 5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPITO), nitric oxide synthase inhibitor S,S'-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea, and inhibitors of the octadecanoid pathway on elicitor-induced NO generation, JA biosynthesis, and hypericin production. Pretreatment of the cells with cPITO and JA biosynthesis inhibitors suppresses not only the elicitor-induced NO generation and JA accumulation but also the elicitor-induced hypericin production, which suggests that both NO and JA are involved in elicitor-induced hypericin biosynthesis. S,S'-1,3-phenylene-bis(1,2-ethanediyl)-bis-isothiourea and cPITO inhibit both elicitor-induced NO generation and JA biosynthesis, while JA biosynthesis inhibitors do not affect the elicitor-induced NO generation, indicating that JA acts downstream of NO generation and that its biosynthesis is regulated by NO. External application of NO via its donor sodium nitroprusside induces hypericin production in the absence of fungal elicitor. Sodium-nitroprusside-induced hypericin production is blocked by JA biosynthesis inhibitors, showing that JA biosynthesis is essential for NO-induced hypericin production. The results demonstrate a causal relationship between elicitor-induced NO generation, JA biosynthesis, and hypericin production in H. perforatum cells and indicate a sequence of signaling events from NO to hypericin production, within which NO mediates the elicitor-induced hypericin biosynthesis at least partially via a JA-dependent signaling pathway.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.066407.

^* Corresponding author; e-mail maojunxu{at}163.com; fax 86–571–88051629.

Received May 31, 2005; returned for revision July 8, 2005; accepted July 8, 2005.

This article has been cited by other articles:

				E. Planchet and W. M. Kaiser Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and biotic NO sources J. Exp. Bot., September 1, 2006; 57(12): 3043 - 3055. [Abstract] [Full Text] [PDF]