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Plant Physiol, April 2000, Vol. 122, pp. 1447-1456
The Sucrose Transporter of Celery. Identification and Expression
during Salt Stress1
Nathalie
Noiraud,
Serge
Delrot, and
Rémi
Lemoine*
Laboratoire de Physiologie et Biochimie Végétales,
Centre National de la Recherche Scientifique Equipe Supérieure
Associée 6161, 40, Avenue du Recteur Pineau, F-86022
Poitiers cedex, France
In celery (Apium
graveolens L.), long-distance transport of reduced carbon
occurs both in the form of sucrose (Suc) and mannitol. The presence of
mannitol has been related to the resistance of celery to salt stress.
To investigate the transport events occurring during salt stress, we
have cloned the H+/Suc transporter of celery AgSUT1
(A. graveolens Suc uptake transport 1) from a mature
leaf cDNA library. The function of the encoded protein was confirmed by
expression in yeast. AgSUT1 is a H+/Suc transporter with a
high affinity for Suc (Km of 139 µM). Another closely related cDNA (AgSUT2)
was also identified. AgSUT1 is mainly expressed in
mature leaves and phloem of petioles, but also in sink organs such as
roots. When celery plants were subjected to salt stress conditions (30 d watering with 300 mM NaCl) favoring mannitol accumulation
(J.D. Everard, R. Gucci, S.C. Kann, J.A. Flore, W.H. Loescher [1994]
Plant Physiol 106: 281-292), AgSUT1 expression was
decreased in all organs, but markedly in roots. The results are
discussed in relation to the physiology of celery.
1
This work was supported by the French Ministry
for Research and Higher Education, the Centre National de la Recherche
Scientifique, and the Region Poitou-Charentes.
*
Corresponding author; e-mail remi.lemoine{at}campus.univ-poitiers.fr;
fax 33-5-49-45-41-86.
© 2000 American Society of Plant Physiologists
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