Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (37)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poirier, Y.
Right arrow Articles by Caldelari, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poirier, Y.
Right arrow Articles by Caldelari, D.
Agricola
Right arrow Articles by Poirier, Y.
Right arrow Articles by Caldelari, D.

Plant Physiol, December 1999, Vol. 121, pp. 1359-1366

Increased Flow of Fatty Acids toward beta -Oxidation in Developing Seeds of Arabidopsis Deficient in Diacylglycerol Acyltransferase Activity or Synthesizing Medium-Chain-Length Fatty Acids1

Yves Poirier,* Giovanni Ventre, and Daniela Caldelari

Institut d'Écologie-Biologie et Physiologie Végétales, Bâtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland

Synthesis of polyhydroxyalkanoates (PHAs) from intermediates of fatty acid beta -oxidation was used as a tool to study fatty acid degradation in developing seeds of Arabidopsis. Transgenic plants expressing a peroxisomal PHA synthase under the control of a napin promoter accumulated PHA in developing seeds to a final level of 0.06 mg g-1 dry weight. In plants co-expressing a plastidial acyl-acyl carrier protein thioesterase from Cuphea lanceolata and a peroxisomal PHA synthase, approximately 18-fold more PHA accumulated in developing seeds. The proportion of 3-hydroxydecanoic acid monomer in the PHA was strongly increased, indicating a large flow of capric acid toward beta -oxidation. Furthermore, expression of the peroxisomal PHA synthase in an Arabidopsis mutant deficient in the enzyme diacylglycerol acyltransferase resulted in a 10-fold increase in PHA accumulation in developing seeds. These data indicate that plants can respond to the inadequate incorporation of fatty acids into triacylglycerides by recycling the fatty acids via beta -oxidation and that a considerable flow toward beta -oxidation can occur even in a plant tissue primarily devoted to the accumulation of storage lipids.

1 This work was supported in part by a grant from the Herbette Foundation. D.C. was a recipient of a fellowship from the Firmenich Foundation (no. 82FI-053519).

* Corresponding author; e-mail yves.poirier{at}ie-bpv.unil.ch; fax 41-21-692-4195.

© 1999 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. Mu, H. Tan, Q. Zheng, F. Fu, Y. Liang, J. Zhang, X. Yang, T. Wang, K. Chong, X.-J. Wang, et al.
LEAFY COTYLEDON1 Is a Key Regulator of Fatty Acid Biosynthesis in Arabidopsis
Plant Physiology, October 1, 2008; 148(2): 1042 - 1054.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Goepfert, J. K. Hiltunen, and Y. Poirier
Identification and Functional Characterization of a Monofunctional Peroxisomal Enoyl-CoA Hydratase 2 That Participates in the Degradation of Even cis-Unsaturated Fatty Acids in Arabidopsis thaliana
J. Biol. Chem., November 24, 2006; 281(47): 35894 - 35903.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Goepfert, C. Vidoudez, E. Rezzonico, J. K. Hiltunen, and Y. Poirier
Molecular Identification and Characterization of the Arabidopsis {Delta}3,5,{Delta}2,4-Dienoyl-Coenzyme A Isomerase, a Peroxisomal Enzyme Participating in the {beta}-Oxidation Cycle of Unsaturated Fatty Acids
Plant Physiology, August 1, 2005; 138(4): 1947 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Y. P. Chia, M. J. Pike, and S. Rawsthorne
Storage oil breakdown during embryo development of Brassica napus (L.)
J. Exp. Bot., May 1, 2005; 56(415): 1285 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Lin, J. E. Cluette-Brown, and H. M. Goodman
The Peroxisome Deficient Arabidopsis Mutant sse1 Exhibits Impaired Fatty Acid Synthesis
Plant Physiology, June 1, 2004; 135(2): 814 - 827.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Moire, E. Rezzonico, S. Goepfert, and Y. Poirier
Impact of Unusual Fatty Acid Synthesis on Futile Cycling through {beta}-Oxidation and on Gene Expression in Transgenic Plants
Plant Physiology, January 1, 2004; 134(1): 432 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
S. Marchesini, N. Erard, T. Glumoff, J. K. Hiltunen, and Y. Poirier
Modification of the Monomer Composition of Polyhydroxyalkanoate Synthesized in Saccharomyces cerevisiae Expressing Variants of the {beta}-Oxidation-Associated Multifunctional Enzyme
Appl. Envir. Microbiol., November 1, 2003; 69(11): 6495 - 6499.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Marchesini and Y. Poirier
Futile Cycling of Intermediates of Fatty Acid Biosynthesis toward Peroxisomal {beta}-Oxidation in Saccharomyces cerevisiae
J. Biol. Chem., August 29, 2003; 278(35): 32596 - 32601.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Jako, A. Kumar, Y. Wei, J. Zou, D. L. Barton, E. M. Giblin, P. S. Covello, and D. C. Taylor
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight
Plant Physiology, June 1, 2001; 126(2): 861 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Allenbach and Y. Poirier
Analysis of the Alternative Pathways for the beta -Oxidation of Unsaturated Fatty Acids Using Transgenic Plants Synthesizing Polyhydroxyalkanoates in Peroxisomes
Plant Physiology, November 1, 2000; 124(3): 1159 - 1168.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists