Plant Physiol. Bio-Rad Microplate Reader
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (85)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wanner, L. A.
Right arrow Articles by Junttila, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wanner, L. A.
Right arrow Articles by Junttila, O.
Agricola
Right arrow Articles by Wanner, L. A.
Right arrow Articles by Junttila, O.

Cold-Induced Freezing Tolerance in Arabidopsis1

Leslie A. Wanner2, * and Olavi Junttila

Institute for Biology, University of Tromsø, N-9037 Tromsø, Norway

Changes in the physiology of plant leaves are correlated with enhanced freezing tolerance and include accumulation of compatible solutes, changes in membrane composition and behavior, and altered gene expression. Some of these changes are required for enhanced freezing tolerance, whereas others are merely consequences of low temperature. In this study we demonstrated that a combination of cold and light is required for enhanced freezing tolerance in Arabidopsis leaves, and this combination is associated with the accumulation of soluble sugars and proline. Sugar accumulation was evident within 2 h after a shift to low temperature, which preceded measured changes in freezing tolerance. In contrast, significant freezing tolerance was attained before the accumulation of proline or major changes in the percentage of dry weight were detected. Many mRNAs also rapidly accumulated in response to low temperature. All of the cold-induced mRNAs that we examined accumulated at low temperature even in the absence of light, when there was no enhancement of freezing tolerance. Thus, the accumulation of these mRNAs is insufficient for cold-induced freezing tolerance.

1   This work was supported in part by the Norwegian Research Council.
2   Present address: Laboratory for Molecular Plant Biology, P.O. Box 5051, Meieribygget, Agricultural University of Norway, N-1432 Aas, Norway.
*   Corresponding author; e-mail leslie.wanner{at}ikb.nlh.no; fax 47-64-94-14-65.

Plant Physiol. (1999) 120: 391-400
Copyright Clearance Center:   0032-0889/99/120//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 J Exp BotHome page
I. Rekarte-Cowie, O. S. Ebshish, K. S. Mohamed, and R. S. Pearce
Sucrose helps regulate cold acclimation of Arabidopsis thaliana
J. Exp. Bot., November 2, 2008; (2008) ern262v1.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
B. Thapa, R. Arora, A. D. Knapp, and E. C. Brummer
Applying Freezing Test to Quantify Cold Acclimation in Medicago truncatula
J. Amer. Soc. Hort. Sci., September 1, 2008; 133(5): 684 - 691.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Kargiotidou, D. Deli, D. Galanopoulou, A. Tsaftaris, and T. Farmaki
Low temperature and light regulate delta 12 fatty acid desaturases (FAD2) at a transcriptional level in cotton (Gossypium hirsutum)
J. Exp. Bot., May 2, 2008; (2008) ern065v1.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
S. H. Hung, C. C. Wang, S. V. Ivanov, V. Alexieva, and C. W. Yu
Repetition of Hydrogen Peroxide Treatment Induces a Chilling Tolerance Comparable to Cold Acclimation in Mung Bean
J. Amer. Soc. Hort. Sci., November 1, 2007; 132(6): 770 - 776.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
A. J. Patton, S. M. Cunningham, J. J. Volenec, and Z. J. Reicher
Differences in Freeze Tolerance of Zoysiagrasses: II. Carbohydrate and Proline Accumulation
Crop Sci., September 1, 2007; 47(5): 2170 - 2181.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C.-H. Dong, X. Hu, W. Tang, X. Zheng, Y. S. Kim, B.-h. Lee, and J.-K. Zhu
A Putative Arabidopsis Nucleoporin, AtNUP160, Is Critical for RNA Export and Required for Plant Tolerance to Cold Stress
Mol. Cell. Biol., December 15, 2006; 26(24): 9533 - 9543.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
E. Ait Barka, J. Nowak, and C. Clement
Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium, Burkholderia phytofirmans Strain PsJN
Appl. Envir. Microbiol., November 1, 2006; 72(11): 7246 - 7252.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Reyes-Diaz, N. Ulloa, A. Zuniga-Feest, A. Gutierrez, M. Gidekel, M. Alberdi, L. J. Corcuera, and L. A. Bravo
Arabidopsis thaliana avoids freezing by supercooling
J. Exp. Bot., November 1, 2006; 57(14): 3687 - 3696.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Mazzucotelli, A. Tartari, L. Cattivelli, and G. Forlani
Metabolism of {gamma}-aminobutyric acid during cold acclimation and freezing and its relationship to frost tolerance in barley and wheat
J. Exp. Bot., November 1, 2006; 57(14): 3755 - 3766.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Maeda, W. Song, T. L. Sage, and D. DellaPenna
Tocopherols Play a Crucial Role in Low-Temperature Adaptation and Phloem Loading in Arabidopsis
PLANT CELL, October 1, 2006; 18(10): 2710 - 2732.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Hannah, D. Wiese, S. Freund, O. Fiehn, A. G. Heyer, and D. K. Hincha
Natural Genetic Variation of Freezing Tolerance in Arabidopsis
Plant Physiology, September 1, 2006; 142(1): 98 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. W.F. Yaish, A. C. Doxey, B. J. McConkey, B. A. Moffatt, and M. Griffith
Cold-Active Winter Rye Glucanases with Ice-Binding Capacity
Plant Physiology, August 1, 2006; 141(4): 1459 - 1472.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. T. Svensson, C. Crosatti, C. Campoli, R. Bassi, A. M. Stanca, T. J. Close, and L. Cattivelli
Transcriptome Analysis of Cold Acclimation in Barley Albina and Xantha Mutants
Plant Physiology, May 1, 2006; 141(1): 257 - 270.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Ciannamea, J. Busscher-Lange, S. de Folter, G. C. Angenent, and R. G. H. Immink
Characterization of the Vernalization Response in Lolium perenne by a cDNA Microarray Approach
Plant Cell Physiol., April 1, 2006; 47(4): 481 - 492.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
C. DHONT, Y. CASTONGUAY, P. NADEAU, G. BELANGER, R. DRAPEAU, S. LABERGE, J.-C. AVICE, and F.-P. CHALIFOUR
Nitrogen Reserves, Spring Regrowth and Winter Survival of Field-grown Alfalfa (Medicago sativa) Defoliated in the Autumn
Ann. Bot., January 1, 2006; 97(1): 109 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Ito, K. Katsura, K. Maruyama, T. Taji, M. Kobayashi, M. Seki, K. Shinozaki, and K. Yamaguchi-Shinozaki
Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice
Plant Cell Physiol., January 1, 2006; 47(1): 141 - 153.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. V. Krutovsky and D. B. Neale
Nucleotide Diversity and Linkage Disequilibrium in Cold-Hardiness- and Wood Quality-Related Candidate Genes in Douglas Fir
Genetics, December 1, 2005; 171(4): 2029 - 2041.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B.-h. Lee, D. A. Henderson, and J.-K. Zhu
The Arabidopsis Cold-Responsive Transcriptome and Its Regulation by ICE1
PLANT CELL, November 1, 2005; 17(11): 3155 - 3175.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
L. V. Savitch, G. Allard, M. Seki, L. S. Robert, N. A. Tinker, N. P. A. Huner, K. Shinozaki, and J. Singh
The Effect of Overexpression of Two Brassica CBF/DREB1-like Transcription Factors on Photosynthetic Capacity and Freezing Tolerance in Brassica napus
Plant Cell Physiol., September 1, 2005; 46(9): 1525 - 1539.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Yano, M. Nakamura, T. Yoneyama, and I. Nishida
Starch-Related {alpha}-Glucan/Water Dikinase Is Involved in the Cold-Induced Development of Freezing Tolerance in Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 837 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Cook, S. Fowler, O. Fiehn, and M. F. Thomashow
From The Cover: A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis
PNAS, October 19, 2004; 101(42): 15243 - 15248.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Takagi, M. Nakamura, H. Hayashi, R. Inatsugi, R. Yano, and I. Nishida
The Leaf-Order-Dependent Enhancement of Freezing Tolerance in Cold-Acclimated Arabidopsis Rosettes is not Correlated with the Transcript Levels of the Cold-Inducible Transcription Factors of CBF/DREB1
Plant Cell Physiol., September 15, 2003; 44(9): 922 - 931.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. R. Tabaei-Aghdaei, R. S. Pearce, and P. Harrison
Sugars regulate cold-induced gene expression and freezing-tolerance in barley cell cultures
J. Exp. Bot., June 1, 2003; 54(387): 1565 - 1575.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Uemura, G. Warren, and P. L. Steponkus
Freezing Sensitivity in the sfr4 Mutant of Arabidopsis Is Due to Low Sugar Content and Is Manifested by Loss of Osmotic Responsiveness
Plant Physiology, April 1, 2003; 131(4): 1800 - 1807.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. E. Vlachonasios, M. F. Thomashow, and S. J. Triezenberg
Disruption Mutations of ADA2b and GCN5 Transcriptional Adaptor Genes Dramatically Affect Arabidopsis Growth, Development, and Gene Expression
PLANT CELL, March 1, 2003; 15(3): 626 - 638.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
C. Dhont, Y. Castonguay, P. Nadeau, G. Belanger, and F.-P. Chalifour
Alfalfa Root Nitrogen Reserves and Regrowth Potential in Response to Fall Harvests
Crop Sci., January 1, 2003; 43(1): 181 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Fowler and M. F. Thomashow
Arabidopsis Transcriptome Profiling Indicates That Multiple Regulatory Pathways Are Activated during Cold Acclimation in Addition to the CBF Cold Response Pathway
PLANT CELL, August 1, 2002; 14(8): 1675 - 1690.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
J. Dionne, Y. Castonguay, P. Nadeau, and Y. Desjardins
Amino Acid and Protein Changes during Cold Acclimation of Green-Type Annual Bluegrass (Poa annua L.) Ecotypes
Crop Sci., November 1, 2001; 41(6): 1862 - 1870.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. J. Gilmour, A. M. Sebolt, M. P. Salazar, J. D. Everard, and M. F. Thomashow
Overexpression of the Arabidopsis CBF3 Transcriptional Activator Mimics Multiple Biochemical Changes Associated with Cold Acclimation
Plant Physiology, December 1, 2000; 124(4): 1854 - 1865.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists