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Nitrate-Ammonium Synergism in Rice. A Subcellular
Flux
Analysis1
Herbert J. Kronzucker*,
M. Yaeesh Siddiqi,
Anthony D.M. Glass, and
Guy J.D. Kirk
Department of Plant Sciences, University of Western Ontario,
London, Ontario, Canada N6A 5B7 (H.J.K.); International Rice Research
Institute, P.O. Box 933, 1099 Manila, Philippines (H.J.K., G.J.D.K.); and Department of Botany, University of British Columbia, Vancouver,
British Columbia, Canada V6T 1Z4 (M.Y.S., A.D.M.G.)
Many reports have shown that plant
growth and yield is superior on mixtures of
NO3 and NH4+ compared
with provision of either N source alone. Despite its clear practical
importance, the nature of this N-source synergism at the cellular level
is poorly understood. In the present study we have used the technique
of compartmental analysis by efflux and the radiotracer 13N
to measure cellular turnover kinetics, patterns of flux partitioning, and cytosolic pool sizes of both NO3 and
NH4+ in seedling roots of rice (Oryza
sativa L. cv IR72), supplied simultaneously with the two N
sources. We show that plasma membrane fluxes for
NH4+, cytosolic NH4+
accumulation, and NH4+ metabolism are enhanced
by the presence of NO3, whereas
NO3 fluxes, accumulation, and metabolism are
strongly repressed by NH4+. However, net N
acquisition and N translocation to the shoot with dual N-source
provision are substantially larger than when NO3 or NH4+ is
provided alone at identical N concentrations.
1
The work reported in this paper was supported by
funds from the "New Frontier" project grant to the International
Rice Research Institute and by a University of Western Ontario grant to
H.J.K.
*
Corresponding author; e-mail kronzuck{at}julian.uwo.ca; fax
1-604-822-6089.
Plant Physiol. (1999) 119: 1041-1046
Copyright Clearance Center: 0032-0889/99/119//06
© 1999 American Society of Plant Physiologists
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