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A Photoperiod-Insensitive Barley Line Contains a Light-Labile
Phytochrome B1
Mamatha Hanumappa2,
Lee H. Pratt,
Marie-Michele Cordonnier-Pratt, and
Gerald F. Deitzer*
Department of Natural Resource Sciences and Landscape
Architecture, University of Maryland, College Park, Maryland 20742 (M.H., G.F.D.); and Department of Botany, University of Georgia,
Athens, Georgia 30602 (L.H.P., M.-M.C.-P.)
Barley
(Hordeum vulgare L.) is a long-day plant whose flowering
is enhanced when the photoperiod is supplemented with far-red light,
and this promotion is mediated by phytochrome. A chemically mutagenized
dwarf cultivar of barley was selected for early flowering time (barley
maturity daylength response [BMDR]-1) and was made isogenic with the
cultivar Shabet (BMDR-8) by backcrossing. BMDR-1 was found to contain
higher levels of both phytochrome A and phytochrome B in the dark on
immunoblots with monoclonal antibodies from oat (Avena
sativa L.) that are specific to different members of the phytochrome gene family. Phytochrome A was light labile in both BMDR-1
and BMDR-8, decreasing to very low levels after 4 d of growth in
the light. Phytochrome B was light stable in BMDR-8, being equal in
both light and darkness. However, phytochrome B became light labile in
BMDR-1 and this destabilization of phytochrome B appeared to make
BMDR-1 insensitive to photoperiod. In addition, both the mutant and the
wild type lacked any significant promotion of flowering in response to
a pulse of far-red light given at the end of day, and the end-of-day,
far-red inhibition of tillering is normal in both, suggesting that
phytochrome B is not involved with these responses in barley.
1
This work was supported in part by the Maryland
Agricultural Experiment Station (project no. MD-L-97) to G.F.D. and by
the U.S. Department of Agriculture National Research Initiative
Competitive Grants Program (no. 93-00939) to L.H.P. and M.-M.C.-P.
2
Current Address: Laboratory for Photoperception
& Signal Transduction, Frontier Research Program, RIKEN,
Wako, Saitama 351-01, Japan.
*
Corresponding author; e-mail gd3{at}umail.umd.edu; fax
1-301-314-9308.
Plant Physiol. (1999) 119: 1033-1040
Copyright Clearance Center: 0032-0889/99/119//08
© 1999 American Society of Plant Physiologists
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