|
Ozone Sensitivity in Hybrid Poplar Is Correlated with a
Lack
of Defense-Gene Activation1
Jennifer Riehl Koch,
Amy J. Scherzer,
Steven M. Eshita, and
Keith
R. Davis*
Forestry Sciences Laboratory, United States Department of
Agriculture Northeastern Forest Experiment Station, Delaware, Ohio
43015-8640 (J.R.K., A.J.S., S.M.E.); and the Department of Plant
Biology and the Plant Biotechnology Center, The Ohio State University,
Columbus, Ohio 43210-1002 (K.R.D.)
Ozone is a major gaseous
pollutant thought to contribute to forest decline. Although the
physiological and morphological responses of forest trees to ozone have
been well characterized, little is known about the molecular basis for
these responses. Our studies compared the response to ozone of
ozone-sensitive and ozone-tolerant clones of hybrid poplar
(Populus maximowizii × Populus
trichocarpa) at the physiological and molecular levels.
Gas-exchange analyses demonstrated clear differences between the
ozone-sensitive clone 388 and the ozone-tolerant clone 245. Although
ozone induced a decrease in photosynthetic rate and stomatal
conductance in both clones, the magnitude of the decrease in stomatal
conductance was significantly greater in the ozone-tolerant clone.
RNA-blot analysis established that ozone-induced mRNA levels for
phenylalanine ammonia-lyase, O-methyltransferase, a
pathogenesis-related protein, and a wound-inducible gene were
significantly higher in the ozone-tolerant than in the ozone-sensitive
plants. Wound- and pathogen-induced levels of these mRNAs were also
higher in the ozone-tolerant compared with the ozone-sensitive plants.
The different physiological and molecular responses to ozone exposure
exhibited by clones 245 and 388 suggest that ozone tolerance involves
the activation of salicylic-acid- and jasmonic-acid-mediated signaling
pathways, which may be important in triggering defense responses
against oxidative stress.
1
This work was supported in part by funds
provided by the U.S. Department of Agriculture, Forest Service,
Northeastern Forest Experiment Station.
*
Corresponding author; e-mail davis.68{at}osu.edu; fax
1-614-292-5379.
Plant Physiol. (1998) 118: 1243-1252
Copyright Clearance Center: 0032-0889/98/118//10
© 1998 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. Cabane, J.-C. Pireaux, E. Leger, E. Weber, P. Dizengremel, B. Pollet, and C. Lapierre
Condensed Lignins Are Synthesized in Poplar Leaves Exposed to Ozone
Plant Physiology,
February 1, 2004;
134(2):
586 - 594.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Pasqualini, C. Piccioni, L. Reale, L. Ederli, G. Della Torre, and F. Ferranti
Ozone-Induced Cell Death in Tobacco Cultivar Bel W3 Plants. The Role of Programmed Cell Death in Lesion Formation
Plant Physiology,
November 1, 2003;
133(3):
1122 - 1134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Vahala, R. Ruonala, M. Keinanen, H. Tuominen, and J. Kangasjarvi
Ethylene Insensitivity Modulates Ozone-Induced Cell Death in Birch
Plant Physiology,
May 1, 2003;
132(1):
185 - 195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Vahala, M. Keinanen, A. Schutzendubel, A. Polle, and J. Kangasjarvi
Differential Effects of Elevated Ozone on Two Hybrid Aspen Genotypes Predisposed to Chronic Ozone Fumigation. Role of Ethylene and Salicylic Acid
Plant Physiology,
May 1, 2003;
132(1):
196 - 205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. M. Pastori, G. Kiddle, J. Antoniw, S. Bernard, S. Veljovic-Jovanovic, P. J. Verrier, G. Noctor, and C. H. Foyer
Leaf Vitamin C Contents Modulate Plant Defense Transcripts and Regulate Genes That Control Development through Hormone Signaling
PLANT CELL,
April 1, 2003;
15(4):
939 - 951.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Overmyer, H. Tuominen, R. Kettunen, C. Betz, C. Langebartels, H. Sandermann , Jr., and J. Kangasjärvi
Ozone-Sensitive Arabidopsis rcd1 Mutant Reveals Opposite Roles for Ethylene and Jasmonate Signaling Pathways in Regulating Superoxide-Dependent Cell Death
PLANT CELL,
October 1, 2000;
12(10):
1849 - 1862.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. P. Constabel, L. Yip, J. J. Patton, and M. E. Christopher
Polyphenol Oxidase from Hybrid Poplar. Cloning and Expression in Response to Wounding and Herbivory
Plant Physiology,
September 1, 2000;
124(1):
285 - 296.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. R. Koch, R. A. Creelman, S. M. Eshita, M. Seskar, J. E. Mullet, and K. R. Davis
Ozone Sensitivity in Hybrid Poplar Correlates with Insensitivity to Both Salicylic Acid and Jasmonic Acid. The Role of Programmed Cell Death in Lesion Formation
Plant Physiology,
June 1, 2000;
123(2):
487 - 496.
[Abstract]
[Full Text]
|
|
|
|
|
