Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jordan, E. T.
Right arrow Articles by Vierstra, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jordan, E. T.
Right arrow Articles by Vierstra, R. D.
Agricola
Right arrow Articles by Jordan, E. T.
Right arrow Articles by Vierstra, R. D.

PLANT PHYSIOLOGY , Vol 115, Issue 2 693-704, Copyright © 1997 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

Characterization of Regions within the N-Terminal 6-Kilodalton Domain of Phytochrome A That Modulate Its Biological Activity

E. T. Jordan, J. M. Marita, R. C. Clough and R. D. Vierstra
Department of Horticulture and the Cell and Molecular Biology Program, University of Wisconsin, Madison, Wisconsin 53706

Phytochrome A (phyA) is a red/far-red (FR) light photoreceptor responsible for initiating numerous light-mediated plant growth and developmental responses, especially in FR light-enriched environments. We previously showed that the first 70 amino acids of the polypeptide contain at least two regions with potentially opposite functions (E.T. Jordan, J.R. Cherry, J.M. Walker, R.D. Vierstra [1996] Plant J 9: 243-257). One region is required for activity and correct apoprotein/chromophore interactions, whereas the second appears to regulate phytochrome activity. We have further resolved these functional regions by analysis of N-terminal deletion and alanine-scanning mutants of oat (Avena sativa) phyA in transgenic tobacco (Nicotiana tabacum). The results indicate that the region involved in chromophore/apoprotein interactions contains two separate segments (residues 25-33 and 50-62) also required for biological activity. The region that regulates phyA activity requires only five adjacent serines (Sers) (residues 8-12). Removal or alteration of these Sers generates a photoreceptor that increases the sensitivity of transgenic seedlings to red and FR light more than intact phyA. Taken together, these data identify three distinct regions in the N-terminal domain necessary for photoreceptor activity, and further define the Ser-rich region as an important site for phyA regulation.


This article has been cited by other articles:


Home page
 Mol PlantHome page
J. Kneissl, T. Shinomura, M. Furuya, and C. Bolle
A Rice Phytochrome A in Arabidopsis: The Role of the N-terminus under red and far-red light
Mol Plant, January 1, 2008; 1(1): 84 - 102.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Kevei, E. Schafer, and F. Nagy
Light-regulated nucleo-cytoplasmic partitioning of phytochromes
J. Exp. Bot., September 27, 2007; (2007) erm145v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. J. Emborg, J. M. Walker, B. Noh, and R. D. Vierstra
Multiple Heme Oxygenase Family Members Contribute to the Biosynthesis of the Phytochrome Chromophore in Arabidopsis
Plant Physiology, March 1, 2006; 140(3): 856 - 868.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-I. Kim, Y. Shen, Y.-J. Han, J.-E. Park, D. Kirchenbauer, M.-S. Soh, F. Nagy, E. Schafer, and P.-S. Song
Phytochrome Phosphorylation Modulates Light Signaling by Influencing the Protein-Protein Interaction
PLANT CELL, October 1, 2004; 16(10): 2629 - 2640.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. J. Casal, S. J. Davis, D. Kirchenbauer, A. Viczian, M. J. Yanovsky, R. C. Clough, S. Kircher, E. T. Jordan-Beebe, E. Schafer, F. Nagy, et al.
The Serine-Rich N-Terminal Domain of Oat Phytochrome A Helps Regulate Light Responses and Subnuclear Localization of the Photoreceptor
Plant Physiology, July 1, 2002; 129(3): 1127 - 1137.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Hanzawa, T. Shinomura, K. Inomata, T. Kakiuchi, H. Kinoshita, K. Wada, and M. Furuya
Structural requirement of bilin chromophore for the photosensory specificity of phytochromes A and B
PNAS, April 2, 2002; 99(7): 4725 - 4729.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Suzuki, S. Takio, I. Yamamoto, and T. Satoh
Characterization of cDNA of the Liverwort Phytochrome Gene, and Phytochrome Involvement in the Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductase Gene Expression in Marchantia paleacea var. diptera
Plant Cell Physiol., June 1, 2001; 42(6): 576 - 582.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K.-C. Yeh and J. C. Lagarias
Eukaryotic phytochromes: Light-regulated serine/threonine protein kinases with histidine kinase ancestry
PNAS, November 10, 1998; 95(23): 13976 - 13981.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists