Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kudlicka, K.
Right arrow Articles by Brown Jr, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kudlicka, K.
Right arrow Articles by Brown Jr, R. M.
Agricola
Right arrow Articles by Kudlicka, K.
Right arrow Articles by Brown Jr, R. M.

PLANT PHYSIOLOGY , Vol 115, Issue 2 643-656, Copyright © 1997 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

Cellulose and Callose Biosynthesis in Higher Plants (I. Solubilization and Separation of (1->3)- and (1->4)-[beta]-Glucan Synthase Activities from Mung Bean)

K. Kudlicka and R. M. Brown Jr
Department of Botany, University of Texas, Austin, Texas 78713-7640

(1->3)- and (1->4)-[beta]-glucan synthase activities from higher plants have been physically separated by gel electrophoresis in nondenaturing conditions. The two glucan synthases show different mobilities in native polyacrylamide gels. Further separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a different polypeptide composition in these synthases. Three polypeptides (64, 54, and 32 kD) seem to be common to both synthase activities, whereas two polypeptides (78 and 38 kD) are associated only with callose synthase activity. Twelve polypeptides (170, 136, 108, 96, 83, 72, 66, 60, 52, 48, 42, and 34 kD) appear to be specifically associated with cellulose synthase activity. The successful separation of (1->3)- and (1->-4)-[beta]-glucan synthase activities was based on the manipulation of digitonin concentrations used in the solubilization of membrane proteins. At low dipitomin concentrations (0.05 and 0.1%), the ratio of the cellulose to callose synthase activity was higher. At higher digitonin (0.5-1%) concentrations, the ratio of the callose to cellulose synthase activity was higher. Rosette-like particles with attached product were observed in samples taken from the top of the stacking gel, where only cellulose was synthesized. Smaller (nonrosette) particles were found in the running gel, where only callose was synthesized. These findings suggest that a higher level of subunit organization is required for in vitro cellulose synthesis in comparison with callose assembly.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. H. Williams
Novelties of the flowering plant pollen tube underlie diversification of a key life history stage
PNAS, August 12, 2008; 105(32): 11259 - 11263.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. C. U. Furch, J. B. Hafke, A. Schulz, and A. J. E. van Bel
Ca2+-mediated remote control of reversible sieve tube occlusion in Vicia faba
J. Exp. Bot., August 1, 2007; 58(11): 2827 - 2838.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
I. M. SAXENA and R. M. BROWN JR
Cellulose Biosynthesis: Current Views and Evolving Concepts
Ann. Bot., July 1, 2005; 96(1): 9 - 21.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
L. Greffe, L. Bessueille, V. Bulone, and H. Brumer
Synthesis, preliminary characterization, and application of novel surfactants from highly branched xyloglucan oligosaccharides
Glycobiology, April 1, 2005; 15(4): 437 - 445.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. S. Dhugga
Plant Golgi cell wall synthesis: From genes to enzyme activities
PNAS, February 8, 2005; 102(6): 1815 - 1816.
[Full Text] [PDF]

Home page
 GlycobiologyHome page
J. K. Fairweather, J. L. K. Him, L. Heux, H. Driguez, and V. Bulone
Structural characterization by 13C-NMR spectroscopy of products synthesized in vitro by polysaccharide synthases using 13C-enriched glycosyl donors: application to a UDP-glucose:(1->3)-{beta}-D-glucan synthase from blackberry (Rubus fruticosus)
Glycobiology, September 1, 2004; 14(9): 775 - 781.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
P. Bhuja, K. McLachlan, J. Stephens, and G. Taylor
Accumulation of 1,3-{beta}-D-glucans, in Response to Aluminum and Cytosolic Calcium in Triticum aestivum
Plant Cell Physiol., May 15, 2004; 45(5): 543 - 549.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
K. S. Dhugga, R. Barreiro, B. Whitten, K. Stecca, J. Hazebroek, G. S. Randhawa, M. Dolan, A. J. Kinney, D. Tomes, S. Nichols, et al.
Guar Seed {beta}-Mannan Synthase Is a Member of the Cellulose Synthase Super Gene Family
Science, January 16, 2004; 303(5656): 363 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. S. Doblin, I. Kurek, D. Jacob-Wilk, and D. P. Delmer
Cellulose Biosynthesis in Plants: from Genes to Rosettes
Plant Cell Physiol., December 15, 2002; 43(12): 1407 - 1420.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Lai-Kee-Him, H. Chanzy, M. Muller, J.-L. Putaux, T. Imai, and V. Bulone
In Vitro Versus in Vivo Cellulose Microfibrils from Plant Primary Wall Synthases: Structural Differences
J. Biol. Chem., September 27, 2002; 277(40): 36931 - 36939.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Paxson-Sowders, C. H. Dodrill, H. A. Owen, and C. A. Makaroff
DEX1, a Novel Plant Protein, Is Required for Exine Pattern Formation during Pollen Development in Arabidopsis
Plant Physiology, December 1, 2001; 127(4): 1739 - 1749.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Hong, A. J. Delauney, and D. P. S. Verma
A Cell Plate-Specific Callose Synthase and Its Interaction with Phragmoplastin
PLANT CELL, April 1, 2001; 13(4): 755 - 768.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
M. S. Doblin, L. De Melis, E. Newbigin, A. Bacic, and S. M. Read
Pollen Tubes of Nicotiana alata Express Two Genes from Different {beta}-Glucan Synthase Families
Plant Physiology, April 1, 2001; 125(4): 2040 - 2052.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. L. Shaw, J. Dumais, and S. R. Long
Cell Surface Expansion in Polarly Growing Root Hairs of Medicago truncatula
Plant Physiology, November 1, 2000; 124(3): 959 - 970.
[Abstract] [Full Text]

Home page
 Plant CellHome page
S. Kimura, W. Laosinchai, T. Itoh, X. Cui, C. R. Linder, and R. M. Brown , Jr.
Immunogold Labeling of Rosette Terminal Cellulose-Synthesizing Complexes in the Vascular Plant Vigna angularis
PLANT CELL, November 1, 1999; 11(11): 2075 - 2086.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists