Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Love, J.
Right arrow Articles by Trewavas, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Love, J.
Right arrow Articles by Trewavas, A. J.
Agricola
Right arrow Articles by Love, J.
Right arrow Articles by Trewavas, A. J.

PLANT PHYSIOLOGY , Vol 115, Issue 1 249-261, Copyright © 1997 by American Society of Plant Biologists

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Ca2+ and Calmodulin Dynamics during Photopolarization in Fucus serratus Zygotes

J. Love, C. Brownlee and A. J. Trewavas
Institute of Cell and Molecular Biology, The University of Edinburgh, Daniel Rutherford Building, King's Buildings, Mayfield Road, Edinburgh EH9 3JH, United Kingdom (J.L., A.J.T.)

The role of Ca2+ in zygote polarization in fucoid algae (Fucus, Ascophyllum, and Pelvetia species) zygote polarization is controversial. Using a local source of Fucus serratus, we established that zygotes form a polar axis relative to unilateral light (photopolarization) between 8 and 14 h after fertilization (AF), and become committed to this polarity at approximately 15 to 18 h AF. We investigated the role of Ca2+, calmodulin, and actin during photopolarization by simultaneously exposing F. serratus zygotes to polarizing light and various inhibitors. Neither removal of Ca2+ from the culture medium or high concentrations of EGTA and LaCl3 had any effect on photopolarization. Bepridil, 3,4,5-trimethoxybenzoic acid 8-(diethylamino) octyl ester, nifedipine, and verapamil, all of which block intracellular Ca2 release, reduced photopolarization from 75 to 30%. The calmodulin antagonists N-(6-aminohexyl)-5-chloro-L-naphthalenesulfonamide and trifluoperazine inhibited photopolarization in all zygotes, whereas N-(6-aminohexyl)-L-naphthalenesulfonamide had no effect. Cytochalasin B, cytochalasin D, and latrunculin B, all of which inhibit actin polymerization, had no effect on photopolarization, but arrested polar axis fixation. The role of calmodulin during polarization was investigated further. Calmodulin mRNA from the closely related brown alga Macrocystis pyrifera was cloned and the protein was expressed in bacteria. Photopolarization was enhanced following microinjections of this recombinant calmodulin into developing zygotes. Confocal imaging of fluorescein isothiocyanate-labeled recombinant calmodulin in photopolarized zygotes showed a homogenous signal distribution at 13 h AF, which localized to the presumptive rhizoid site at 15 h AF.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
F. Corellou, S. M. B. Coelho, F.-Y. Bouget, and C. Brownlee
Spatial re-organisation of cortical microtubules in vivo during polarisation and asymmetric division of Fucus zygotes
J. Cell Sci., June 15, 2005; 118(12): 2723 - 2734.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Sun, S. Basu, S. R. Brady, R. L. Luciano, and G. K. Muday
Interactions between Auxin Transport and the Actin Cytoskeleton in Developmental Polarity of Fucus distichus Embryos in Response to Light and Gravity
Plant Physiology, May 1, 2004; 135(1): 266 - 278.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C Bachewich and I. Heath
Radial F-actin arrays precede new hypha formation in Saprolegnia: implications for establishing polar growth and regulating tip morphogenesis
J. Cell Sci., June 8, 2000; 111(14): 2005 - 2016.
[Abstract] [PDF]

Home page
 Plant CellHome page
B. C. Gibbon, D. R. Kovar, and C. J. Staiger
Latrunculin B Has Different Effects on Pollen Germination and Tube Growth
PLANT CELL, December 1, 1999; 11(12): 2349 - 2364.
[Abstract] [Full Text]

Home page
 Plant CellHome page
D. Sanders, C. Brownlee, and J. F. Harper
Communicating with Calcium
PLANT CELL, April 1, 1999; 11(4): 691 - 706.
[Full Text]

Home page
 DevelopmentHome page
L Alessa and D. Kropf
F-actin marks the rhizoid pole in living Pelvetia compressa zygotes
Development, January 1, 1999; 126(1): 201 - 209.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
R Pu and K. Robinson
Cytoplasmic calcium gradients and calmodulin in the early development of the fucoid alga Pelvetia compressa
J. Cell Sci., January 11, 1998; 111(21): 3197 - 3207.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Goddard, N. F. H. Manison, D. Tomos, and C. Brownlee
Elemental propagation of calcium signals in response-specific patterns determined by environmental stimulus strength
PNAS, February 15, 2000; 97(4): 1932 - 1937.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists