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PLANT PHYSIOLOGY , Vol 114, Issue 3 969-979, Copyright © 1997 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Sex Determination in Dioecious Silene Iatifolia (Effects of the Y Chromosome and the Parasitic Smut Fungus (Ustilago violacea) on Gene Expression during Flower Development)
C. P. Scutt, Y. Li, S. E. Robertson, M. E. Willis and P. M. Gilmartin
Centre for Plant Biochemistry and Biotechnology, The University of Leeds, Leeds LS2 9JT, United Kingdom
We have embarked on a molecular cloning approach to the investigation of
sex determination in Silene Iatifolia Poiret, a dioecious plant species
with morphologically distinguishable sex chromosomes. One of our key
objectives was to define a range of genes that are up-regulated in male
plants in response to Y chromosome sex-determination genes. Here we present
the characterization of eight male-specific cDNA sequences and classify
these according to their expression dynamics to provide a range of
molecular markers for dioecious male flower development. Genetically female
S. latifolia plants undergo a partial sex reversal in response to infection
by the parasitic smut fungus Ustilago violacea. This phenomenon has been
exploited in these studies; male-specific cDNAs have been further
categorized as inducible or noninducible in female plants by smut fungus
infection. Analysis of the organ-specific expression of male-specific
probes in male and female flowers has also identified a gene that is
regulated in a sex-specific manner in nonreproductive floral tissues common
to both male and female plants. This observation provides, to our
knowledge, the first molecular marker for dominant effects of the Y
chromosome in nonreproductive floral organs.
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