Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bohland, C.
Right arrow Articles by Grambow, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bohland, C.
Right arrow Articles by Grambow, H. J.
Agricola
Right arrow Articles by Bohland, C.
Right arrow Articles by Grambow, H. J.

PLANT PHYSIOLOGY , Vol 114, Issue 2 679-685, Copyright © 1997 by American Society of Plant Biologists

PLANT-MICROBE AND PLANT-INSECT INTERACTIONS

Differential Induction of Lipoxygenase Isoforms in Wheat upon Treatment with Rust Fungus Elicitor, Chitin Oligosaccharides, Chitosan, and Methyl Jasmonate

C. Bohland, T. Balkenhohl, G. Loers, I. Feussner and H. J. Grambow
Institut fur Biologie III, Pflanzenphysiologie, Technische Hochschule Aachen, D-52056 Aachen, Germany

A glycopeptide elicitor prepared from germ tubes of the rust fungus Puccinia graminis Pers. f. sp. tritici Erikss. & Henn (Pgt), as well as chitin oligosaccharides, chitosan, and methyl jasmonate (MJ) stimulated lipoxygenase (LOX) activity (E.C. 1.13.11.12) in wheat (Triticum aestivum) leaves. Immunoblot analysis using anti-LOX antibodies revealed the induction of 92- and 103-kD LOX species after Pgt elicitor treatment. In contrast, MJ treatment led to a significant increase of a 100-kD LOX species, which was also detected at lower levels in control plants. The effects of chitin oligomers and chitosan resembled those caused by MJ. In conjunction with other observations the results suggest that separate reaction cascades exist, and that jasmonates may not be involved in Pgt elicitor action. LOX-92 appears to be mainly responsible for the increase in LOX activity after Pgt elicitor treatment because its appearance on western blots coincided with high LOX activity in distinct anion-exchange chromatography fractions. It is most active at pH 5.5 to 6.0, and product formation from linoleic and [alpha]-linolenic acid is clearly in favor of the 9-LOOHs. It is interesting that a 92-kD LOX species, which seems to correspond to the Pgt elicitor-induced LOX species, was also detected in rust-inoculated leaves.


This article has been cited by other articles:


Home page
 J Exp BotHome page
B.-E. Amborabe, J. Bonmort, P. Fleurat-Lessard, and G. Roblin
Early events induced by chitosan on plant cells
J. Exp. Bot., June 1, 2008; 59(9): 2317 - 2324.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Nemchenko, S. Kunze, I. Feussner, and M. Kolomiets
Duplicate maize 13-lipoxygenase genes are differentially regulated by circadian rhythm, cold stress, wounding, pathogen infection, and hormonal treatments
J. Exp. Bot., November 1, 2006; 57(14): 3767 - 3779.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Klarzynski, B. Plesse, J.-M. Joubert, J.-C. Yvin, M. Kopp, B. Kloareg, and B. Fritig
Linear beta -1,3 Glucans Are Elicitors of Defense Responses in Tobacco
Plant Physiology, November 1, 2000; 124(3): 1027 - 1038.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
M. V. Kolomiets, H. Chen, R. J. Gladon, E.J. Braun, and D. J. Hannapel
A Leaf Lipoxygenase of Potato Induced Specifically by Pathogen Infection
Plant Physiology, November 1, 2000; 124(3): 1121 - 1130.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. Gobel, I. Feussner, A. Schmidt, D. Scheel, J. Sanchez-Serrano, M. Hamberg, and S. Rosahl
Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells
J. Biol. Chem., February 23, 2001; 276(9): 6267 - 6273.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists