|
PLANT PHYSIOLOGY , Vol 113, Issue 4 1447-1455, Copyright © 1997 by American Society of Plant Biologists
|
CELL BIOLOGY AND SIGNAL TRANSDUCTION |
The Organization of the Actin Cytoskeleton in Vertical and Graviresponding Primary Roots of Maize
E. B. Blancaflor and K. H. Hasenstein
Department of Biology, University of Southwestern Louisiana, Lafayette, Louisiana 70504
To determine whether actin microfilament (MF) organization is correlated
with differential elongation, primary roots of Zea mays cv Merit maintained
vertically or reoriented horizontally for 15 to 120 min were stained with
rhodamine phalloidin and examined with a confocal microscope. Root
curvature was measured with a computer-controlled video digitizer. In
vertical roots bundles of MFs in the elongation and maturation zone were
oriented parallel to the longitudinal axis of cells. MFs in the vascular
parenchyma cells were more abundant than in the cortex and epidermis.
Epidermal and proendodermal cells in the meristematic region contained
transverse cortical MFs. The organization of MFs of graviresponding roots
was similar to that of vertical roots. Application of cytochalasin B or
cytochalasin D resulted in extensive disruption of MFs in the cortex and
epidermis, but only partially affected MFs in the stele. Despite the
cytochalasin B-induced depolymerization of MFs, gravicurvature exceeded
that of controls. In contrast, the auxin transport inhibitor N-1
naphthylphthalamic acid suppressed root curvature but had no observable
effect on the integrity of the MFs. The data indicate that MFs may not be
involved in the graviresponse of maize roots.
This article has been cited by other articles:
|
|
|
|
 
Z. Ma and K. H. Hasenstein
The Onset of Gravisensitivity in the Embryonic Root of Flax
Plant Physiology,
January 1, 2006;
140(1):
159 - 166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Limbach, J. Hauslage, C. Schafer, and M. Braun
How to Activate a Plant Gravireceptor. Early Mechanisms of Gravity Sensing Studied in Characean Rhizoids during Parabolic Flights
Plant Physiology,
October 1, 2005;
139(2):
1030 - 1040.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-B. Li, X.-P. Fan, X.-L. Wang, L. Cai, and W.-C. Yang
The Cotton ACTIN1 Gene Is Functionally Expressed in Fibers and Participates in Fiber Elongation
PLANT CELL,
March 1, 2005;
17(3):
859 - 875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Hou, D. R. Mohamalawari, and E. B. Blancaflor
Enhanced Gravitropism of Roots with a Disrupted Cap Actin Cytoskeleton
Plant Physiology,
March 1, 2003;
131(3):
1360 - 1373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Wyatt, A. M. Rashotte, M. J. Shipp, D. Robertson, and G. K. Muday
Mutations in the Gravity Persistence Signal Loci in Arabidopsis Disrupt the Perception and/or Signal Transduction of Gravitropic Stimuli
Plant Physiology,
November 1, 2002;
130(3):
1426 - 1435.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamamoto and J. Z. Kiss
Disruption of the Actin Cytoskeleton Results in the Promotion of Gravitropism in Inflorescence Stems and Hypocotyls of Arabidopsis
Plant Physiology,
February 1, 2002;
128(2):
669 - 681.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Yoder, H.-q. Zheng, P. Todd, and L. A. Staehelin
Amyloplast Sedimentation Dynamics in Maize Columella Cells Support a New Model for the Gravity-Sensing Apparatus of Roots
Plant Physiology,
February 1, 2001;
125(2):
1045 - 1060.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. B. Blancaflor, D. L. Jones, and S. Gilroy
Alterations in the Cytoskeleton Accompany Aluminum-Induced Growth Inhibition and Morphological Changes in Primary Roots of Maize
Plant Physiology,
September 1, 1998;
118(1):
159 - 172.
[Abstract]
[Full Text]
|
|
|
|
|
