Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kalaitzis, P.
Right arrow Articles by Tucker, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kalaitzis, P.
Right arrow Articles by Tucker, M. L.
Agricola
Right arrow Articles by Kalaitzis, P.
Right arrow Articles by Tucker, M. L.

PLANT PHYSIOLOGY , Vol 113, Issue 4 1303-1308, Copyright © 1997 by American Society of Plant Biologists

GENE REGULATION AND MOLECULAR GENETICS

Three Different Polygalacturonases Are Expressed in Tomato Leaf and Flower Abscission, Each with a Different Temporal Expression Pattern

P. Kalaitzis, T. Solomos and M. L. Tucker
Plant Molecular Biology Laboratory, United States Department of Agriculture, Agriculture Research Service, Building 006, BARC-West, Beltsville, Maryland 20705 (P.K., M.L.T.)

Abscission, or organ separation, is accompanied by a marked increase in hydrolases, which are responsible for the degradation of the middle lamella and the loosening of the primary cell wall surrounding cells in the separation layer. We recently reported on the cloning of a tomato (Lycopersicon esculentum) polygalacturonase (PG) cDNA, TAPG1, expressed during leaf and flower abscission. In addition to TAPG1, we have cloned two more PG cDNAs (TAPG2 and TAPG4) that are also expressed during leaf and flower abscission. The peptide sequences for the three abscission PGs are relatively similar (76-93% identity) yet different from the those of tomato fruit PG (38-41% identity). None of the three abscission PG mRNAs are expressed in fruit, stems, petioles, or anthers of fully open flowers. An RNase protection assay revealed that all three PGs are expressed in leaf and flower abscission zones and in pistils of fully open flowers. TAPG4 mRNA is detected much earlier than TAPG1 and TAPG2 mRNA during both leaf and flower abscission.


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Agusti, P. Merelo, M. Cercos, F. R. Tadeo, and M. Talon
Ethylene-induced differential gene expression during abscission of citrus leaves
J. Exp. Bot., July 1, 2008; 59(10): 2717 - 2733.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C.-Z. Jiang, F. Lu, W. Imsabai, S. Meir, and M. S. Reid
Silencing polygalacturonase expression inhibits tomato petiole abscission
J. Exp. Bot., March 2, 2008; (2008) ern023v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. H. Gonzalez-Carranza, K. A. Elliott, and J. A. Roberts
Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana
J. Exp. Bot., October 10, 2007; (2007) erm222v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. A. Butenko, G.-E. Stenvik, V. Alm, B. Saether, S. E. Patterson, and R. B. Aalen
Ethylene-dependent and -independent pathways controlling floral abscission are revealed to converge using promoter::reporter gene constructs in the ida abscission mutant
J. Exp. Bot., November 1, 2006; 57(14): 3627 - 3637.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
I. Y. Abdurakhmonov, A. A. Abdullaev, S. Saha, Z. T. Buriev, D. Arslanov, Z. Kuryazov, G. T. Mavlonov, S. M. Rizaeva, U. K. Reddy, J. N. Jenkins, et al.
Simple Sequence Repeat Marker Associated with a Natural Leaf Defoliation Trait in Tetraploid Cotton
J. Hered., November 1, 2005; 96(6): 644 - 653.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Wu and J. K. Burns
A {beta}-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis)
J. Exp. Bot., July 1, 2004; 55(402): 1483 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Capodicasa, D. Vairo, O. Zabotina, L. McCartney, C. Caprari, B. Mattei, C. Manfredini, B. Aracri, J. Benen, J. P. Knox, et al.
Targeted Modification of Homogalacturonan by Transgenic Expression of a Fungal Polygalacturonase Alters Plant Growth
Plant Physiology, July 1, 2004; 135(3): 1294 - 1304.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Orozco-Cardenas and C. A. Ryan
Polygalacturonase {beta}-Subunit Antisense Gene Expression in Tomato Plants Leads to a Progressive Enhanced Wound Response and Necrosis in Leaves and Abscission of Developing Flowers
Plant Physiology, October 1, 2003; 133(2): 693 - 701.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. G. Atkinson, R. Schroder, I. C. Hallett, D. Cohen, and E. A. MacRae
Overexpression of Polygalacturonase in Transgenic Apple Trees Leads to a Range of Novel Phenotypes Involving Changes in Cell Adhesion
Plant Physiology, May 1, 2002; 129(1): 122 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. H. Gonzalez-Carranza, C. A. Whitelaw, R. Swarup, and J. A. Roberts
Temporal and Spatial Expression of a Polygalacturonase during Leaf and Flower Abscission in Oilseed Rape and Arabidopsis
Plant Physiology, February 1, 2002; 128(2): 534 - 543.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Federici, C. Caprari, B. Mattei, C. Savino, A. Di Matteo, G. De Lorenzo, F. Cervone, and D. Tsernoglou
Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein)
PNAS, October 25, 2001; (2001) 231473698.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S.-B. Hong, R. Sexton, and M. L. Tucker
Analysis of Gene Promoters for Two Tomato Polygalacturonases Expressed in Abscission Zones and the Stigma
Plant Physiology, July 1, 2000; 123(3): 869 - 882.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
K. A. Hadfield and A. B. Bennett
Polygalacturonases: Many Genes in Search of a Function
Plant Physiology, June 1, 1998; 117(2): 337 - 343.
[Full Text]

Home page
 Plant Physiol.Home page
K. A. Hadfield, J. K.C. Rose, D. S. Yaver, R. M. Berka, and A. B. Bennett
Polygalacturonase Gene Expression in Ripe Melon Fruit Supports a Role for Polygalacturonase in Ripening-Associated Pectin Disassembly
Plant Physiology, June 1, 1998; 117(2): 363 - 373.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
F. D. Degan, R. Child, I. Svendsen, and P. Ulvskov
The Cleavable N-terminal Domain of Plant Endopolygalacturonases from Clade B May Be Involved in a Regulated Secretion Mechanism
J. Biol. Chem., September 14, 2001; 276(38): 35297 - 35304.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Federici, C. Caprari, B. Mattei, C. Savino, A. Di Matteo, G. De Lorenzo, F. Cervone, and D. Tsernoglou
Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein)
PNAS, November 6, 2001; 98(23): 13425 - 13430.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. A. Whitelaw, N. N. Lyssenko, L. Chen, D. Zhou, A. K. Mattoo, and M. L. Tucker
Delayed Abscission and Shorter Internodes Correlate with a Reduction in the Ethylene Receptor LeETR1 Transcript in Transgenic Tomato
Plant Physiology, March 1, 2002; 128(3): 978 - 987.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists