PLANT PHYSIOLOGY , Vol 113, Issue 2 611-619, Copyright © 1997 by American Society of Plant Biologists

GENE REGULATION AND MOLECULAR GENETICS

The Sorghum Photoperiod Sensitivity Gene, Ma3, Encodes a Phytochrome B

K. L. Childs, F. R. Miller, M. M. Cordonnier-Pratt, L. H. Pratt, P. W. Morgan and J. E. Mullet
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128 (K.L.C., J.E.M.)

The Ma3 gene is one of six genes that regulate the photoperiodic sensitivity of flowering in sorghum (Sorghum bicolor [L.] Moench). The ma3R mutation of this gene causes a phenotype that is similar to plants that are known to lack phytochrome B, and ma3R sorghum lacks a 123-kD phytochrome that predominates in light-grown plants and that is present in non-ma3R plants. A population segregating for Ma3 and ma3R was created and used to identify two randomly amplified polymorphic DNA markers linked to Ma3. These two markers were cloned and mapped in a recombinant inbred population as restriction fragment length polymorphisms. cDNA clones of PHYA and PHYC were cloned and sequenced from a cDNA library prepared from green sorghum leaves. Using a genome-walking technique, a 7941-bp partial sequence of PHYB was determined from genomic DNA from ma3R sorghum. PHYA, PHYB, and PHYC all mapped to the same linkage group. The Ma3- linked markers mapped with PHYB more than 121 centimorgans from PHYA and PHYC. A frameshift mutation resulting in a premature stop codon was found in the PHYB sequence from ma3R sorghum. Therefore, we conclude that the Ma3 locus in sorghum is a PHYB gene that encodes a 123-kD phytochrome.

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