Plant Physiol. Bio-Rad Microplate Reader
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eckardt, N. A.
Right arrow Articles by Ogren, W. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eckardt, N. A.
Right arrow Articles by Ogren, W. L.
Agricola
Right arrow Articles by Eckardt, N. A.
Right arrow Articles by Ogren, W. L.

PLANT PHYSIOLOGY , Vol 113, Issue 2 575-586, Copyright © 1997 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

Growth and Photosynthesis under High and Low Irradiance of Arabidopsis thaliana Antisense Mutants with Reduced Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Content

N. A. Eckardt, G. W. Snyder, A. R. Portis Jr and W. L. Ogren
Department of Plant Biology, University of Illinois, Urbana, Illinois (N.A.E.)

Photosynthesis and growth to maturity of antisense ribulose-1,5- bisphosphate carboxylase/oxygenase (Rubisco) activase Arabidopsis thaliana with reduced concentrations of activase relative to wild-type (Wt) plants were measured under low (200 [mu]mol m-2 s-1) and high (600 [mu]mol m-2 s-1) photosynthetic photon flux density growing conditions. Both growth and photosynthesis were significantly reduced in an Arabidopsis clone (R100) with 30 to 40% Wt activase, an effect that was more pronounced in high light. The aboveground biomass of the antisense clone R100 reached 80% of Wt under low light and 65% of Wt under high light. Decreased growth in the antisense plants was attributed to reduced relative rates of growth and leaf area expansion early in development; all plants attained similar values of relative rates of growth and leaf elongation by 21 d after planting. Reductions in photosynthesis were attributed to decreased Rubisco activation in the antisense plants. Rubisco constituted about 40% of total soluble protein in both Wt and clone R100 under both light regimes. Activase content was 5% and 1.4% of total soluble protein in Wt and clone R100, respectively, and also was unaffected by growth irradiance. The stoichiometry of Rubisco to activase was estimated at 20 Rubisco active sites per activase tetramer in Wt Arabidopsis and 60 to 80 in the transgenic clone R100. We conclude that Wt Arabidopsis does not contain Rubisco activase in great excess of the amount required for optimal growth.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Mitra and I. T. Baldwin
Independently Silencing Two Photosynthetic Proteins in Nicotiana attenuata Has Different Effects on Herbivore Resistance
Plant Physiology, October 1, 2008; 148(2): 1128 - 1138.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. E. Salvucci
Association of Rubisco activase with chaperonin-60{beta}: a possible mechanism for protecting photosynthesis during heat stress
J. Exp. Bot., May 1, 2008; 59(7): 1923 - 1933.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Hendrickson, R. Sharwood, M. Ludwig, S. M. Whitney, M. R. Badger, and S. von Caemmerer
The effects of Rubisco activase on C4 photosynthesis and metabolism at high temperature
J. Exp. Bot., May 1, 2008; 59(7): 1789 - 1798.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. A. J. Parry, A. J. Keys, P. J. Madgwick, A. E. Carmo-Silva, and P. J. Andralojc
Rubisco regulation: a role for inhibitors
J. Exp. Bot., May 1, 2008; 59(7): 1569 - 1580.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Makino and R. F. Sage
Temperature Response of Photosynthesis in Transgenic Rice Transformed with 'Sense' or 'Antisense' rbcS
Plant Cell Physiol., October 1, 2007; 48(10): 1472 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X.-G. Zhu, E. de Sturler, and S. P. Long
Optimizing the Distribution of Resources between Enzymes of Carbon Metabolism Can Dramatically Increase Photosynthetic Rate: A Numerical Simulation Using an Evolutionary Algorithm
Plant Physiology, October 1, 2007; 145(2): 513 - 526.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. E. Salvucci, B. P. DeRidder, and A. R. Portis Jr
Effect of activase level and isoform on the thermotolerance of photosynthesis in Arabidopsis
J. Exp. Bot., November 1, 2006; 57(14): 3793 - 3799.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S.-H. JIN, J. HONG, X.-Q. LI, and D.-A. JIANG
Antisense Inhibition of Rubisco Activase Increases Rubisco Content and Alters the Proportion of Rubisco Activase in Stroma and Thylakoids in Chloroplasts of Rice Leaves
Ann. Bot., May 1, 2006; 97(5): 739 - 744.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Li, M. E. Salvucci, and A. R. Portis Jr.
Two Residues of Rubisco Activase Involved in Recognition of the Rubisco Substrate
J. Biol. Chem., July 1, 2005; 280(26): 24864 - 24869.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Kim and A. R. Portis Jr
Temperature Dependence of Photosynthesis in Arabidopsis Plants with Modifications in Rubisco Activase and Membrane Fluidity
Plant Cell Physiol., March 1, 2005; 46(3): 522 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. von Caemmerer, L. Hendrickson, V. Quinn, N. Vella, A.G. Millgate, and R.T. Furbank
Reductions of Rubisco Activase by Antisense RNA in the C4 Plant Flaveria bidentis Reduces Rubisco Carbamylation and Leaf Photosynthesis
Plant Physiology, February 1, 2005; 137(2): 747 - 755.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Dhingra, A. R. Portis Jr., and H. Daniell
Enhanced translation of a chloroplast-expressed RbcS gene restores small subunit levels and photosynthesis in nuclear RbcS antisense plants
PNAS, April 20, 2004; 101(16): 6315 - 6320.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. V. Pollock, S. L. Colombo, D. L. Prout Jr., A. C. Godfrey, and J. V. Moroney
Rubisco Activase Is Required for Optimal Photosynthesis in the Green Alga Chlamydomonas reinhardtii in a Low-CO2 Atmosphere
Plant Physiology, December 1, 2003; 133(4): 1854 - 1861.
[Abstract] [Full Text]

Home page
 Plant Cell PhysiolHome page
X.-H. Zhang, R. G. Ewy, J. M. Widholm, and A. R. Portis Jr.
Complementation of the Nuclear Antisense rbcS-Induced Photosynthesis Deficiency by Introducing an rbcS Gene into the Tobacco Plastid Genome
Plant Cell Physiol., November 15, 2002; 43(11): 1302 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
R. F. Sage
How Terrestrial Organisms Sense, Signal, and Respond to Carbon Dioxide
Integr. Comp. Biol., July 1, 2002; 42(3): 469 - 480.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Zhang, R. P. Kallis, R. G. Ewy, and A. R. Portis Jr.
Light modulation of Rubisco in Arabidopsis requires a capacity for redox regulation of the larger Rubisco activase isoform
PNAS, February 14, 2002; (2002) 42529999.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. P. Kallis, R. G. Ewy, and A. R. Portis Jr.
Alteration of the Adenine Nucleotide Response and Increased Rubisco Activation Activity of Arabidopsis Rubisco Activase by Site-Directed Mutagenesis
Plant Physiology, July 1, 2000; 123(3): 1077 - 1086.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Zhang and A. R. Portis Jr.
Mechanism of light regulation of Rubisco: A specific role for the larger Rubisco activase isoform involving reductive activation by thioredoxin-f
PNAS, August 3, 1999; 96(16): 9438 - 9443.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. D. Law and S. J. Crafts-Brandner
Inhibition and Acclimation of Photosynthesis to Heat Stress Is Closely Correlated with Activation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
Plant Physiology, May 1, 1999; 120(1): 173 - 182.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
U. Feller, S. J. Crafts-Brandner, and M. E. Salvucci
Moderately High Temperatures Inhibit Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (Rubisco) Activase-Mediated Activation of Rubisco
Plant Physiology, February 1, 1998; 116(2): 539 - 546.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Zhang, R. P. Kallis, R. G. Ewy, and A. R. Portis Jr.
From the Cover: Light modulation of Rubisco in Arabidopsis requires a capacity for redox regulation of the larger Rubisco activase isoform
PNAS, March 5, 2002; 99(5): 3330 - 3334.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists