Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Covello, P. S.
Right arrow Articles by Reed, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Covello, P. S.
Right arrow Articles by Reed, D. W.
Agricola
Right arrow Articles by Covello, P. S.
Right arrow Articles by Reed, D. W.

PLANT PHYSIOLOGY , Vol 111, Issue 1 223-226, Copyright © 1996 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

Functional Expression of the Extraplastidial Arabidopsis thaliana Oleate Desaturase Gene (FAD2) in Saccharomyces cerevisiae

P. S. Covello and D. W. Reed
National Research Council, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine [delta]12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and [delta]9,[delta]12-hexadecadienoic acid by gas chromatography-mass spectrometry.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y.-Y. Kim, D.-Y. Kim, D. Shim, W.-Y. Song, J. Lee, J. I. Schroeder, S. Kim, N. Moran, and Y. Lee
Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast
J. Biol. Chem., June 6, 2008; 283(23): 15893 - 15902.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
H. Yazawa, H. Iwahashi, Y. Kamisaka, K. Kimura, T. Aki, K. Ono, and H. Uemura
Heterologous Production of Dihomo-{gamma}-Linolenic Acid in Saccharomyces cerevisiae
Appl. Envir. Microbiol., November 1, 2007; 73(21): 6965 - 6971.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Meesapyodsuk, D. W. Reed, P. S. Covello, and X. Qiu
Primary Structure, Regioselectivity, and Evolution of the Membrane-bound Fatty Acid Desaturases of Claviceps purpurea
J. Biol. Chem., July 13, 2007; 282(28): 20191 - 20199.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Meesapyodsuk, J. Balsevich, D. W. Reed, and P. S. Covello
Saponin Biosynthesis in Saponaria vaccaria. cDNAs Encoding beta-Amyrin Synthase and a Triterpene Carboxylic Acid Glucosyltransferase
Plant Physiology, February 1, 2007; 143(2): 959 - 969.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
S. Rodriguez-Vargas, A. Sanchez-Garcia, J. M. Martinez-Rivas, J. A. Prieto, and F. Randez-Gil
Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces cerevisiae to Freezing and Salt Stress
Appl. Envir. Microbiol., January 1, 2007; 73(1): 110 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Sasata, D. W. Reed, M. C. Loewen, and P. S. Covello
Domain Swapping Localizes the Structural Determinants of Regioselectivity in Membrane-bound Fatty Acid Desaturases of Caenorhabditis elegans
J. Biol. Chem., September 17, 2004; 279(38): 39296 - 39302.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. B. Cahoon and A. J. Kinney
Dimorphecolic Acid Is Synthesized by the Coordinate Activities of Two Divergent {Delta}12-Oleic Acid Desaturases
J. Biol. Chem., March 26, 2004; 279(13): 12495 - 12502.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. M. Dyer, D. C. Chapital, J.-C. W. Kuan, R. T. Mullen, C. Turner, T. A. McKeon, and A. B. Pepperman
Molecular Analysis of a Bifunctional Fatty Acid Conjugase/Desaturase from Tung. Implications for the Evolution of Plant Fatty Acid Diversity
Plant Physiology, December 1, 2002; 130(4): 2027 - 2038.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Broadwater, E. Whittle, and J. Shanklin
Desaturation and Hydroxylation. RESIDUES 148 AND 324 OF ARABIDOPSIS FAD2, IN ADDITION TO SUBSTRATE CHAIN LENGTH, EXERT A MAJOR INFLUENCE IN PARTITIONING OF CATALYTIC SPECIFICITY
J. Biol. Chem., May 3, 2002; 277(18): 15613 - 15620.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
A. C. Bruner, S. Jung, A. G. Abbott, and G. L. Powell
The Naturally Occurring High Oleate Oil Character in Some Peanut Varieties Results from Reduced Oleoyl-PC Desaturase Activity from Mutation of Aspartate 150 to Asparagine
Crop Sci., March 1, 2001; 41(2): 522 - 526.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Qiu, D. W. Reed, H. Hong, S. L. MacKenzie, and P. S. Covello
Identification and Analysis of a Gene from Calendula officinalis Encoding a Fatty Acid Conjugase
Plant Physiology, February 1, 2001; 125(2): 847 - 855.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
D. W. Reed, U. A. Schafer, and P. S. Covello
Characterization of the Brassica napus Extraplastidial Linoleate Desaturase by Expression in Saccharomyces cerevisiae
Plant Physiology, March 1, 2000; 122(3): 715 - 720.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P. Broun, J. Shanklin, E. Whittle, and C. Somerville
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
Science, November 13, 1998; 282(5392): 1315 - 1317.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. B. Cahoon, K. G. Ripp, S. E. Hall, and A. J. Kinney
Formation of Conjugated Delta 8,Delta 10-Double Bonds by Delta 12-Oleic-acid Desaturase-related Enzymes. BIOSYNTHETIC ORIGIN OF CALENDIC ACID
J. Biol. Chem., January 19, 2001; 276(4): 2637 - 2643.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1996 by the American Society of Plant Biologists