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PLANT PHYSIOLOGY , Vol 111, Issue 1 203-213, Copyright © 1996 by American Society of Plant Biologists
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PLANT-MICROBE AND PLANT-INSECT INTERACTIONS |
Differential Localization of Carbohydrate Epitopes in Plant Cell Walls in the Presence and Absence of Arbuscular Mycorrhizal Fungi
R. Balestrini, M. G. Hahn, A. Faccio, K. Mendgen and P. Bonfante
Dipartimento di Biologia Vegetale, Universita di Torino, Viale Mattioli 25, 10125, Torino, Italy (R.B., P.B.)
Two monoclonal antibodies (McAbs) generated against rhamnogalacturonan I
and characterized as specific for a terminal [alpha]-(1->2)-linked
fucosyl-containing epitope (CCRC-M1) and for an arabinosylated
[beta]-(1,6)-galactan epitope (CCRC-M7) were used in immunogold experiments
to determine the distribution of the epitopes in four plants. Allium
porrum, Zea mays, Trifolium repens, and Nicotiana tabacum plants were
chosen as representatives of monocots and dicots with different wall
structures. Analyses were performed on root tissues in the presence and
absence of arbuscular mycorrhizal fungi. A differential localization of the
two cell wall epitopes was found between tissues and between species: for
example, in leek, CCRC-M1 labeled epidermal and hypodermal cells, whereas
CCRC-M7 labeled cortical cells only. Clover walls were labeled by both
McAbs, whereas maize and tobacco were only labeled by CCRC-M7. In the
presence of the arbuscular mycorrhizal fungi, labeling was additionally
found in an apoplastic compartment typical of the symbiosis (the interface)
occurring around the intracellular hyphae. Epitopes binding both McAbs were
found in the interfacial material, and their distribution mirrored the
pattern found in the host cell wall. These findings demonstrate that the
composition of the interface zone in a fungus-plant symbiosis reflects the
composition of the wall of the host cell.
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