PLANT PHYSIOLOGY , Vol 110, Issue 4 1231-1237, Copyright © 1996 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Molecular Characterization of Two Brassica napus Pollen-Expressed Genes Encoding Putative Arabinogalactan Proteins
J. Gerster, S. Allard and L. S. Robert
Plant Research Centre, Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6
Two highly homologous cDNA clones, Sta 39-3 and Sta 39-4, corresponding to
mRNAs highly expressed in Brassica napus cv Wester stamens were isolated by
differential screening and characterized. Northern blot and in situ
analyses demonstrated that Sta 39-3 and Sta 39-4 transcripts accumulate in
pollen following the first pollen mitosis and are abundant at pollen
maturity, thus identifying them as "late" pollen genes. Sta 39-3 and Sta
39-4 belong to a small gene family. Their predicted proteins share
similarities with those deduced from recently isolated cDNAs encoding
arabinogalactan proteins, which include a similar molecular mass
(approximately 13 kD); high levels of alanine, proline, serine, and
threonine, which are interspersed throughout the protein with no obvious
repetitive motif; low levels of cysteine, histidine, tryptophan, and
tyrosine; and hydrophobic N- and C-terminal ends. The Sta 39-3 and Sta 39-4
proteins may play a role in pollen germination and tube growth.