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PLANT PHYSIOLOGY , Vol 107, Issue 1 25-31, Copyright © 1995 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Developmental and Hormonal Regulation of Rice [alpha]-Amylase(RAmy1A)-gusA Fusion Genes in Transgenic Rice Seeds
K. Itoh, J. Yamaguchi, N. Huang, R. L. Rodriguez, T. Akazawa and K. Shimamoto
Plantech Research Institute, 1000 Kamoshida, Aoba-ku,Yokohama 227, Japan (K.I., K.S.)
Transgenic seeds of rice (Oryza sativa L.) were used to investigate
temporal, spatial, and hormonal regulation of a rice [alpha]-amylase gene,
RAmy1A. Two overlapping segments of the RAmy1A promoter were fused to the
coding region of the bacterial reporter gene, gusA. The resulting
promoter-gusA fusions, pE4/GUS (-232 to +31) and pH4/GUS (-748 to +31),
were used separately to transform rice protoplasts. [beta]-Glucuronidase
(GUS) activity was detected in germinated transgenic seeds, although the
two constructs showed no significant difference in timing or location of
GUS expression. Both constructs first expressed GUS in the scutellar
epithelium and then in the aleurone layer. Aleurone expression of GUS
activity was strongly induced when embryoless half-seeds were treated with
gibberellic acid. GUS expression in the aleurone layer was also suppressed
by abscisic acid. These results indicate that the 5[prime] regulatory
region from -232 to +31 is sufficient for temporal, spatial, and hormonal
regulation of RAmy1A gene expression.
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