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PLANT PHYSIOLOGY , Vol 103, Issue 4 1355-1359, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Biochemical Evidence for the Role of the Waxy Protein from Pea (Pisum sativum L.) as a Granule-Bound Starch Synthase

M. N. Sivak, M. Wagner and J. Preiss
Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824

Proteins were solubilized from starch extracted from developing pea (Pisum sativum L.) embryos and chromatography of these proteins on a Mono-Q column separated two peaks of starch synthase activity. The major activity peak comprised more than 80% of the total activity. This fraction contained only the Waxy protein, as shown by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate followed by staining for proteins or by immunoblot. A 77-kD polypeptide associated with the starch granules and presumed by others to be a starch synthase could not be detected in any of the active fractions. The native molecular weight of the solubilized starch synthase was 59,600 [plus or minus] 1700 as determined by sucrose density gradient. It is concluded that in pea seeds the Waxy protein and the starch synthase bound to the granule are the same protein.


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A. Mérida, J. M. Rodríguez-Galán, C. Vincent, and J. M. Romero
Expression of the Granule-Bound Starch Synthase I (Waxy) Gene from Snapdragon Is Developmentally and Circadian Clock Regulated
Plant Physiology, June 1, 1999; 120(2): 401 - 410.
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Copyright © 1993 by the American Society of Plant Biologists