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PLANT PHYSIOLOGY , Vol 102, Issue 2 537-539, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

The Extraction and Assay of 1-Kestose:Sucrose Fructosyl Transferase from Leaves of Wheat

C. J. Pollock and T. L. Housley
Environmental Biology Department, Agricultural and Food Research Council Institute for Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, Wales, United Kingdom (C.J.P.)

Isolating the enzymes responsible for fructan synthesis in plants has been hampered by unsuitable assays used during purification. It is believed that there are two enzymes necessary for fructan synthesis in higher plants, one initiating synthesis utilizing sucrose as donor and the other elaborating the polymer using fructan oligomers as donor. In this paper, a rapid quantitative assay is described to measure the latter fructosyl transfer. The activity was absent from leaves that were not synthesizing fructan. Activity in crude extracts showed a hyperbolic dependence upon sucrose concentration. Activity against 1-kestose showed a pronounced optimum, suggesting that self-transfer also occurred.




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Copyright © 1993 by the American Society of Plant Biologists