Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ginsburg, S.
Right arrow Articles by Matile, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ginsburg, S.
Right arrow Articles by Matile, P.
Agricola
Right arrow Articles by Ginsburg, S.
Right arrow Articles by Matile, P.

PLANT PHYSIOLOGY , Vol 102, Issue 2 521-527, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Identification of Catabolites of Chlorophyll-Porphyrin in Senescent Rape Cotyledons

S. Ginsburg and P. Matile
Department of Plant Biology, University of Zurich, Zollikerstrasse 107, CH-8008 Zurich, Switzerland

Developing shoots of rape seedlings (Brassica napus L.) were excised and fed with 4-[14C]5-aminolevulinic acid to label the pyrroles in chlorophyll (Chl) synthesized during the final phase of expansion and greening of the cotyledons. About 80% of 14C taken up into the cotyledons was incorporated into Chl. The subsequent incubation of labeled shoots in permanent darkness caused the rapid loss of labeled Chl while increasing proportions of 14C appeared in the fraction of water-soluble compounds. Reversed-phase high performance liquid chromatography resolved three nonfluorescent polar catabolites of Chl-porphyrin that were progressively accumulated as senescence advanced. At intermediate stages of senescence, the cotyledons contained a fluorescent radio-active derivative of Chl that was also detectable, together with traces of other putative fluorescent catabolites, in isolated senescent chloroplasts. The nonfluorescent catabolites, identified by means of radiolabeling, were also found to accumulate in attached cotyledons senescing under photoperiod; under these conditions, one of the compounds, NCC-1, was particularly abundant. The catabolites of rape exhibited the same ultraviolet spectra, characterized by a maximum at 320 nm, as a previously reported secoporphinoid catabolite from barley (B. Krautler, B. Jaun, W. Amrein, K. Bortlik, M. Schellenberg, P. Matile [1992] Plant Physiol Biochem 30: 333-346). Different polarities suggest, however, that the structures may be different. A terminology for Chl catabolites is proposed because present knowledge suggests that a large number of different structures results from species-specific processing of breakdown products and may require a suitable nomenclature.


This article has been cited by other articles:


Home page
 Plant CellHome page
A. Pruzinska, I. Anders, S. Aubry, N. Schenk, E. Tapernoux-Luthi, T. Muller, B. Krautler, and S. Hortensteiner
In Vivo Participation of Red Chlorophyll Catabolite Reductase in Chlorophyll Breakdown
PLANT CELL, January 1, 2007; 19(1): 369 - 387.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Pruzinska, G. Tanner, S. Aubry, I. Anders, S. Moser, T. Muller, K.-H. Ongania, B. Krautler, J.-Y. Youn, S. J. Liljegren, et al.
Chlorophyll Breakdown in Senescent Arabidopsis Leaves. Characterization of Chlorophyll Catabolites and of Chlorophyll Catabolic Enzymes Involved in the Degreening Reaction
Plant Physiology, September 1, 2005; 139(1): 52 - 63.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Pruzinska, G. Tanner, I. Anders, M. Roca, and S. Hortensteiner
Chlorophyll breakdown: Pheophorbide a oxygenase is a Rieske-type iron-sulfur protein, encoded by the accelerated cell death 1 gene
PNAS, December 9, 2003; 100(25): 15259 - 15264.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Hortensteiner and U. Feller
Nitrogen metabolism and remobilization during senescence
J. Exp. Bot., April 15, 2002; 53(370): 927 - 937.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Hinder, M. Schellenberg, S. Rodoni, S. Ginsburg, E. Vogt, E. Martinoia, P. Matile, and S. Hortensteiner
How Plants Dispose of Chlorophyll Catabolites. DIRECTLY ENERGIZED UPTAKE OF TETRAPYRROLIC BREAKDOWN PRODUCTS INTO ISOLATED VACUOLES
J. Biol. Chem., November 1, 1996; 271(44): 27233 - 27236.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1993 by the American Society of Plant Biologists