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PLANT PHYSIOLOGY , Vol 102, Issue 2 445-458, Copyright © 1993 by American Society of Plant Biologists

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Structure of Ten Free N-Glycans in Ripening Tomato Fruit (Arabinose Is a Constituent of a Plant N-Glycan)

B. Priem, R. Gitti, C. A. Bush and K. C. Gross
Horticultural Crops Quality Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Building 002, Beltsville, Maryland 20705 (B.P., K.C.G.)

The concentration-dependent stimulatory and inhibitory effect of N-glycans on tomato (Lycopersicon esculentum Mill.) fruit ripening was recently reported (B. Priem and K.C. Gross [1992] Plant Physiol 98: 399-401). We report here the structure of 10 free N-glycans in mature green tomatoes. N-Glycans were purified from fruit pericarp by ethanolic extraction, desalting, concanavalin A-Sepharose chromatography, and amine-bonded silica high performance liquid chromatography. N-Glycan structures were determined using 500 MHz 1H-nuclear magnetic resonance spectroscopy, fast atom bombardment mass spectrometry, and glycosyl linkage methylation analysis by gas chromatography-mass spectrometry. A novel arabinosyl-containing N-glycan, Man[alpha]1->6(Ara[alpha]1-> 2)Man[beta]1-> 4GlcNAc[beta]1->4(Fuc[alpha]1->3)GlcNAc, was purified from a retarded concanavalin A fraction. The location of the arabinosyl residue was the same as the xylosyl residue in complex N-glycans. GlcNAc[5[prime]]Man3(Xyl)GlcNAc(Fuc)GlcNAc and GlcNAc[5[prime]]Man2GlcNAc(Fuc)GlcNAc were also purified from the weakly retained fraction. The oligomannosyl N-glycans Man5GlcNAc, Man6GlcNAc, Man7GlcNAc, and Man8GlcNAc were purified from a strongly retained concanavalin A fraction. The finding of free Man5GlcNAc in situ was important physiologically because previously we had described it as a promoter of tomato ripening when added exogenously. Mature green pericarp tissue contained more than 1 [mu]g of total free N-glycan/g fresh weight. Changes in N-glycan composition were determined during ripening by comparing glycosyl and glycosyl-linkage composition of oligosaccharidic extracts from fruit at different developmental stages. N-Glycans were present in pericarp tissue at all stages of development. However, the amount increased during ripening, as did the relative amount of xylosyl-containing N-glycans.


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