|
PLANT PHYSIOLOGY , Vol 102, Issue 1 303-312, Copyright © 1993 by American Society of Plant Biologists
|
DEVELOPMENT AND GROWTH REGULATION |
Characterization of a Gene That Is Expressed Early in Somatic Embryogenesis of Daucus carota
E. S. Wurtele, H. Wang, S. Durgerian, B. J. Nikolau and T. H. Ulrich
Department of Botany (E.S.W., H.W., S.D.), Department of Food Science and Human Nutrition (E.S.W., S.D.), and Department of Biochemistry and Biophysics (B.J.N.)., Iowa State University, Ames, Iowa 50011
The EMB-1 mRNA of carrot (Daucus carota) was isolated as an embryo abundant
cDNA clone (T.H. Ulrich, E.S. Wurtele, B.J. Nikolau [1990] Nucleic Acids
Res 18: 2826). Northern analyses of RNA isolated from embryos, cultured
cells, and a variety of vegetative organs indicate that the EMB-1 mRNA
specifically accumulates in embryos, beginning at the early stages of
embryo development. In situ hybridization with both zygotic and somatic
embryos show that the EMB-1 mRNA begins to accumulate at low levels
throughout globular embryos. Accumulation of EMB-1 mRNA increases and
becomes more localized as embryos mature; in torpedo embryos, EMB-1 mRNA
preferentially accumulates in the meristematic regions, particularly the
procambium. The similarity in distribution of EMB-1 mRNA in both zygotic
and somatic embryos indicates that much of the spatial pattern of
expression of the emb-1 gene is dependent on the developmental program of
the carrot embryo and does not require maternal or endosperm factors. The
EMB-1 protein (relative molecular weight 9910) is a very hydrophilic
protein that is a member of a class of highly conserved proteins (typified
also by the Em protein of wheat and the Lea D 19 protein of cotton) that
may be ubiquitous among angiosperm embryos but whose functions are as yet
unknown. The carrot genome appears to contain one or two copies of the
emb-1 gene. A 1313-base pair DNA fragment of the carrot genome containing
the emb-1 gene was isolated and sequenced. The gene is interrupted by a
single intron of 99 base pairs. Primer extension experiments identify two
EMB-1 mRNAs, differing by 6 bases at their 5[prime] ends that are
transcribed from this gene.
This article has been cited by other articles:
|
|
|
|
 
M. Battaglia, Y. Olvera-Carrillo, A. Garciarrubio, F. Campos, and A. A. Covarrubias
The Enigmatic LEA Proteins and Other Hydrophilins
Plant Physiology,
September 1, 2008;
148(1):
6 - 24.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Umehara, S. Ogita, H. Sasamoto, H. Koshino, T. Asami, S. Fujioka, S. Yoshida, and H. Kamada
Identification of a Novel factor, Vanillyl Benzyl Ether, Which Inhibits Somatic Embryogenesis of Japanese Larch (Larix leptolepis Gordon)
Plant Cell Physiol.,
March 1, 2005;
46(3):
445 - 453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Takahata, M. Takeuchi, M. Fujita, J. Azuma, H. Kamada, and F. Sato
Isolation of Putative Glycoprotein Gene from Early Somatic Embryo of Carrot and its Possible Involvement in Somatic Embryo Development
Plant Cell Physiol.,
November 15, 2004;
45(11):
1658 - 1668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Boutilier, R. Offringa, V. K. Sharma, H. Kieft, T. Ouellet, L. Zhang, J. Hattori, C.-M. Liu, A. A. M. van Lammeren, B. L. A. Miki, et al.
Ectopic Expression of BABY BOOM Triggers a Conversion from Vegetative to Embryonic Growth
PLANT CELL,
August 1, 2002;
14(8):
1737 - 1749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Vicient, G. Hull, J. Guilleminot, M. Devic, and M. Delseny
Differential expression of the Arabidopsis genes coding for Em-like proteins
J. Exp. Bot.,
July 1, 2000;
51(348):
1211 - 1220.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Schmidt, F Guzzo, M. Toonen, and S. de Vries
A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos
Development,
January 5, 1997;
124(10):
2049 - 2062.
[Abstract]
[PDF]
|
|
|
|
|
