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PLANT PHYSIOLOGY , Vol 102, Issue 1 219-225, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Endopolygalacturonase in Apples (Malus domestica) and Its Expression during Fruit Ripening

Q. Wu, M. Szakacs-Dobozi, M. Hemmat and G. Hrazdina
Institute of Food Science (Q.W., M.S.-D., G.H.), and Department of Horticulture (M.H.), Cornell University, Geneva, New York 14456-0462

The activity of polygalacturonase (PG) has been detected in ripe McIntosh apples (Malus domestica Borkh. cv McIntosh) both by enzyme activity measurement and immunoblotting using an anti-tomato-PG antibody preparation. PG activity increased during fruit ripening and remained steady, or decreased slightly, after 5 months of controlled atmospheric storage. The enzyme had a relative molecular weight of 45,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 56,000 to 61,000 when determined by gel filtration. Viscosity and reducing end group measurements with a commercial pectin preparation showed that the enzyme is endo acting. In RNA and DNA blot hybridization experiments, a full-length tomato PG cDNA hybridized with the apple RNA and DNA, showing the identity of genes encoding the activity of the enzyme in tomato and apple.


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Copyright © 1993 by the American Society of Plant Biologists