PLANT PHYSIOLOGY , Vol 101, Issue 3 1081-1088, Copyright © 1993 by American Society of Plant Biologists
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PLANT-MICROBE INTERACTIONS |
Sensitivity to an Ethylene Biosynthesis-Inducing Endoxylanase in Nicotiana tabacum L. cv Xanthi Is Controlled by a Single Dominant Gene
B. A. Bailey, R. F. Korcak and J. D. Anderson
Weed Science Laboratory (B.A.B., J.D.A.), and Fruit Laboratory (R.F.K.), Beltsville Agricultural Research Center (West), Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705
The ethylene biosynthesis-inducing xylanase (EIX) is known to be a potent
elicitor of ethylene biosynthesis and other responses when applied to leaf
tissue of Nicotiana tabacum L. cv Xanthi. In contrast, leaf tissue of the
tobacco cultivar Hicks was insensitive to EIX at concentrations 100-fold
higher than was needed to elicit responses from Xanthi. Cell-suspension
cultures of Xanthi and Hicks showed similar differences in sensitivity to
EIX. Equivalent levels of ethylene production were elicited in leaf discs
of both cultivars after treatment with CuSO4. The F1 and Xanthi backcross
progeny of Hicks and Xanthi crosses were all sensitive to EIX, whereas the
F2 and Hicks backcross progeny segregated for sensitivity to EIX.
Individual plants from the F2 and Hicks backcross that were insensitive to
EIX produced only insensitive progeny when they were self-pollinated.
Progeny from sensitive plants either segregated for sensitivity to EIX or
produced all sensitive progeny (an F2 plant). Sensitivity to EIX is
controlled by a single dominant gene, based on chi-square analysis of
segregation ratios.
