Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Bilsen, DGJL.
Right arrow Articles by Hoekstra, F. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Bilsen, DGJL.
Right arrow Articles by Hoekstra, F. A.
Agricola
Right arrow Articles by van Bilsen, DGJL.
Right arrow Articles by Hoekstra, F. A.

PLANT PHYSIOLOGY , Vol 101, Issue 2 675-682, Copyright © 1993 by American Society of Plant Biologists

ENVIRONMENTAL AND STRESS PHYSIOLOGY

Decreased Membrane Integrity in Aging Typha latifolia L.Pollen (Accumulation of Lysolipids and Free Fatty Acids)

DGJL. van Bilsen and F. A. Hoekstra
Department of Plant Physiology, Agricultural University Wageningen, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands

Aging of cattail (Typha latifolia L.) pollen was studied at 24[deg]C under conditions of 40 and 75% relative humidity (RH). The decline of viability coincides with increased leakage at imbibition; both processes develop much faster at the higher humidity condition. During aging phospholipids are deesterified and free fatty acids (FFAs) and lysophospholipids (LPLs) accumulate, again, much more rapidly at 75% RH than at 40% RH. The fatty acid composition of the remaining phospholipids hardly changes during aging, which suggests limited involvement of lipid peroxidation in the degradation process. Tests with phospholipase A2 revealed that the saturated fatty acids occur at the sn-1 position of the glycerol backbone of the phospholipids. The fatty acid composition of the LPLs is similar to that of the phospholipids from which they were formed, indicating that the deesterification occurs at random. This favors involvement of free radicals instead of phospholipases in the deesterification process. Liposome studies were carried out to characterize components in the lipid fraction that might account for the leakage associated with aging. Entrapped carboxyfluorescein leaked much more from liposomes when they were partly made up from total lipids from aged pollen than from nonaged pollen. The components causing the leakage were found in both the polar and the neutral lipid fractions. Further purification and subsequent interchanging of the FFAs and LPLs between extracts from aged and nonaged pollen revealed that in neutral lipid extracts the FFAs are entirely responsible for the leakage, whereas in the phospholipid fraction the LPLs are largely responsible for the leakage. The leakage from the liposomes is not caused by fusion. We suggest that the observed loss of viability and increased leakage during aging are due to the nonenzymic accumulation of FFAs and LPLs in the pollen membranes.


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Song and S. Tachibana
Loss of viability of tomato pollen during long-term dry storage is associated with reduced capacity for translating polyamine biosynthetic enzyme genes after rehydration
J. Exp. Bot., December 7, 2007; (2007) erm280v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Brehelin, E. H. Meyer, J.-P. de Souris, G. Bonnard, and Y. Meyer
Resemblance and Dissemblance of Arabidopsis Type II Peroxiredoxins: Similar Sequences for Divergent Gene Expression, Protein Localization, and Activity
Plant Physiology, August 1, 2003; 132(4): 2045 - 2057.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
J. H. Crowe, A. E. Oliver, and F. Tablin
Is There a Single Biochemical Adaptation to Anhydrobiosis?
Integr. Comp. Biol., July 1, 2002; 42(3): 497 - 503.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Leprince and F. A. Hoekstra
The Responses of Cytochrome Redox State and Energy Metabolism to Dehydration Support a Role for Cytoplasmic Viscosity in Desiccation Tolerance
Plant Physiology, December 1, 1998; 118(4): 1253 - 1264.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. Buitink, M. M.A.E. Claessens, M. A. Hemminga, and F. A. Hoekstra
Influence of Water Content and Temperature on Molecular Mobility and Intracellular Glasses in Seeds and Pollen
Plant Physiology, October 1, 1998; 118(2): 531 - 541.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Buitink, O. Leprince, M. A. Hemminga, and F. A. Hoekstra
Molecular mobility in the cytoplasm: An approach to describe and predict lifespan of dry germplasm
PNAS, February 29, 2000; 97(5): 2385 - 2390.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1993 by the American Society of Plant Biologists