This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Krishna, P. Articles by Das, A. Search for Related Content PubMed PubMed Citation Articles by Krishna, P. Articles by Das, A. Agricola Articles by Krishna, P. Articles by Das, A.

Molecular Biology and Gene Regulation

Structure and Light-Induced Expression of a Small Heat-Shock Protein Gene of Pharbitis nil¹

Department of Biochemistry, University of Minnesota, St. Paul, Minnesota 55108, Plant Molecular Genetics Institute, University of Minnesota, St. Paul, Minnesota 55108

To isolate genes that are regulated by a photoperiod that promotes flowering in Pharbitis nil, a cDNA library representing mRNA of induced cotyledons was screened by differential hybridization. The DNA sequence of one cDNA clone isolated by this approach, clone 12L, showed homology to plant small heat-shock protein (hsp) genes. P. nil genomic clones hybridizing to clone 12L were isolated, and the DNA sequences of two P. nil small hsp (shsp) genes, shsp-1 and shsp-2, were determined. The derived amino acid sequences of shsp-1 and shsp-2 showed maximum homology to the 17.9-kD soybean hsp, a member of the class II cytoplasmic hsps found in plants. A study of the expression of shsp-1 and shsp-2 genes by RNase protection assay indicated that shsp-1 is induced by photoperiod, by light treatment of dark-grown P. nil seedlings, and by heat shock, and that shsp-2 is induced only by heat shock. Analysis of the sequences of the nontranscribed region indicates that both genes contain multiple heat-shock elements. The shsp-1 gene, in addition, contains sequences homologous to the GT-1-binding site, which may play a role in its light-regulated expression.

³ Present address: School of Biological Sciences, University of Nebraska, Lincoln, NE 68588.

¹ This work was supported by grants from the National Institutes of Health (GM 42557) and the Agricultural Experiment Station of the University of Minnesota. P.K. was a postdoctoral fellow of the Plant Molecular Genetics Institute of the University of Minnesota.

This article has been cited by other articles:

				K. Sage-Ono, M. Ono, H. Harada, and H. Kamada Accumulation of a Clock-Regulated Transcript during Flower-Inductive Darkness in Pharbitis nil Plant Physiology, April 1, 1998; 116(4): 1479 - 1485. [Abstract] [Full Text]

				R. Carranco, C. Almoguera, and J. Jordano A Plant Small Heat Shock Protein Gene Expressed during Zygotic Embryogenesis but Noninducible by Heat Stress J. Biol. Chem., October 24, 1997; 272(43): 27470 - 27475. [Abstract] [Full Text] [PDF]