This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ludevid, D. Articles by Chrispeels, M. J. Search for Related Content PubMed PubMed Citation Articles by Ludevid, D. Articles by Chrispeels, M. J. Agricola Articles by Ludevid, D. Articles by Chrispeels, M. J.

Development and Growth Regulation

The Expression Pattern of the Tonoplast Intrinsic Protein -TIP in Arabidopsis thaliana Is Correlated with Cell Enlargement ¹

Department of Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0116

The vacuolar membrane (tonoplast) contains an abundant intrinsic protein with six membrane-spanning domains that is encoded by a small gene family. Different isoforms of tonoplast intrinsic protein (TIP) are expressed in different tissues or as a result of specific signals. Using promoter--glucuronidase (GUS) fusions and in situ hybridization, we have examined the expression of -TIP in Arabidopsis thaliana. GUS staining of plants transformed with promoter-GUS fusions showed that -TIP gene expression is high in recently formed tissues of young roots. In the shoot, -TIP gene expression was highest in the vascular bundles of stems and petioles, as well as in the stipules and in the receptacle of the flower. No GUS activity was detected in root or shoot meristems or in older tissues, suggesting temporal control of -TIP gene expression associated with cell elongation and/or differentiation. In situ hybridization carried out with whole seedlings confirmed that in root tips, -TIP mRNA was present only in the zone of cell elongation just behind the apical meristem. In seedling shoots, mRNA abundance was also found to be correlated with cell expansion. These results indicate that -TIP may be expressed primarily at the time when the large central vacuoles are being formed during cell enlargement.

³ Present address: Laboratoire de Biologie Cellulaire, INRA, Route de Saint-Cyr F-78026 Versailles, France.

¹ Supported by a grant from the United States Department of Agriculture (to M.J.C.), a European Molecular Biology Organization fellowship (to H.H.), and a fellowship from the Provincial Government of Catalonia (to D.L.).

This article has been cited by other articles:

				J. Traas Whole-Mount In Situ Hybridization of RNA Probes to Plant Tissues CSH Protocols, February 1, 2008; 2008(3): pdb.prot4944 - pdb.prot4944. [Abstract] [Full Text]

				P. R. Hunter, C. P. Craddock, S. Di Benedetto, L. M. Roberts, and L. Frigerio Fluorescent Reporter Proteins for the Tonoplast and the Vacuolar Lumen Identify a Single Vacuolar Compartment in Arabidopsis Cells Plant Physiology, December 1, 2007; 145(4): 1371 - 1382. [Abstract] [Full Text] [PDF]

				A. Olbrich, S. Hillmer, G. Hinz, P. Oliviusson, and D. G. Robinson Newly Formed Vacuoles in Root Meristems of Barley and Pea Seedlings Have Characteristics of Both Protein Storage and Lytic Vacuoles Plant Physiology, December 1, 2007; 145(4): 1383 - 1394. [Abstract] [Full Text] [PDF]

				A. Berr and I. Schubert Interphase Chromosome Arrangement in Arabidopsis thaliana Is Similar in Differentiated and Meristematic Tissues and Shows a Transient Mirror Symmetry After Nuclear Division Genetics, June 1, 2007; 176(2): 853 - 863. [Abstract] [Full Text] [PDF]

				C. Vander Willigen, O. Postaire, C. Tournaire-Roux, Y. Boursiac, and C. Maurel Expression and Inhibition of Aquaporins in Germinating Arabidopsis Seeds Plant Cell Physiol., September 1, 2006; 47(9): 1241 - 1250. [Abstract] [Full Text] [PDF]

				M. O'Brien, S.-C. Chantha, A. Rahier, and D. P. Matton Lipid Signaling in Plants. Cloning and Expression Analysis of the Obtusifoliol 14{alpha}-Demethylase from Solanum chacoense Bitt., a Pollination- and Fertilization-Induced Gene with Both Obtusifoliol and Lanosterol Demethylase Activity Plant Physiology, October 1, 2005; 139(2): 734 - 749. [Abstract] [Full Text] [PDF]

				D. A. Eisenbarth and A. R. Weig Dynamics of aquaporins and water relations during hypocotyl elongation in Ricinus communis L. seedlings J. Exp. Bot., July 1, 2005; 56(417): 1831 - 1842. [Abstract] [Full Text] [PDF]

				H. JAVOT and C. MAUREL The Role of Aquaporins in Root Water Uptake Ann. Bot., September 1, 2002; 90(3): 301 - 313. [Abstract] [Full Text] [PDF]

				N. Miyamoto, T. Ookawa, H. Takahashi, and T. Hirasawa Water Uptake and Hydraulic Properties of Elongating Cells in Hydrotropically Bending Roots of Pisum sativum L. Plant Cell Physiol., April 15, 2002; 43(4): 393 - 401. [Abstract] [Full Text] [PDF]

				I. Hakman and P. Oliviusson High expression of putative aquaporin genes in cells with transporting and nutritive functions during seed development in Norway spruce (Picea abies) J. Exp. Bot., April 1, 2002; 53(369): 639 - 649. [Abstract] [Full Text] [PDF]

				V. T. Ciavatta, R. Morillon, G. S. Pullman, M. J. Chrispeels, and J. Cairney An Aquaglyceroporin Is Abundantly Expressed Early in the Development of the Suspensor and the Embryo Proper of Loblolly Pine Plant Physiology, December 1, 2001; 127(4): 1556 - 1567. [Abstract] [Full Text] [PDF]

				F. Siefritz, A. Biela, M. Eckert, B. Otto, N. Uehlein, and R. Kaldenhoff The tobacco plasma membrane aquaporin NtAQP1 J. Exp. Bot., October 1, 2001; 52(363): 1953 - 1957. [Abstract] [Full Text] [PDF]

				U. Johanson, M. Karlsson, I. Johansson, S. Gustavsson, S. Sjovall, L. Fraysse, A. R. Weig, and P. Kjellbom The Complete Set of Genes Encoding Major Intrinsic Proteins in Arabidopsis Provides a Framework for a New Nomenclature for Major Intrinsic Proteins in Plants Plant Physiology, August 1, 2001; 126(4): 1358 - 1369. [Abstract] [Full Text] [PDF]

				E. Martinoia, A. Massonneau, and N. Frangne Transport Processes of Solutes across the Vacuolar Membrane of Higher Plants Plant Cell Physiol., November 1, 2000; 41(11): 1175 - 1186. [Abstract] [Full Text] [PDF]

				H. Koltai and D. M. Bird High Throughput Cellular Localization of Specific Plant mRNAs by Liquid-Phase in Situ Reverse Transcription-Polymerase Chain Reaction of Tissue Sections Plant Physiology, August 1, 2000; 123(4): 1203 - 1212. [Abstract] [Full Text]

				W. M. Gray, J. C. del Pozo, L. Walker, L. Hobbie, E. Risseeuw, T. Banks, W. L. Crosby, M. Yang, H. Ma, and M. Estelle Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana Genes & Dev., July 1, 1999; 13(13): 1678 - 1691. [Abstract] [Full Text]

				F. Marty Plant Vacuoles PLANT CELL, April 1, 1999; 11(4): 587 - 600. [Full Text]

				D. C. Boyes, J. Nam, and J. L. Dangl The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response PNAS, December 22, 1998; 95(26): 15849 - 15854. [Abstract] [Full Text] [PDF]

				M. Sidler, P. Hassa, S. Hasan, C. Ringli, and R. Dudler Involvement of an ABC Transporter in a Developmental Pathway Regulating Hypocotyl Cell Elongation in the Light PLANT CELL, October 1, 1998; 10(10): 1623 - 1636. [Abstract] [Full Text]

				F. Chaumont, F. Barrieu, E. M. Herman, and M. J. Chrispeels Characterization of a Maize Tonoplast Aquaporin Expressed in Zones of Cell Division and Elongation Plant Physiology, August 1, 1998; 117(4): 1143 - 1152. [Abstract] [Full Text]

				F. Barrieu, F. Chaumont, and M. J. Chrispeels High Expression of the Tonoplast Aquaporin ZmTIP1 in Epidermal and Conducting Tissues of Maize Plant Physiology, August 1, 1998; 117(4): 1153 - 1163. [Abstract] [Full Text]

				R. J. Cowling, Y. Kamiya, H. Seto, and N. P. Harberd Gibberellin Dose-Response Regulation of GA4 Gene Transcript Levels in Arabidopsis Plant Physiology, August 1, 1998; 117(4): 1195 - 1203. [Abstract] [Full Text]

				F. Chaumont, W. F. Loomis, and M. J. Chrispeels Expression of an Arabidopsis plasma membrane aquaporin in Dictyostelium results in hypoosmotic sensitivity and developmental abnormalities PNAS, June 10, 1997; 94(12): 6202 - 6209. [Abstract] [Full Text] [PDF]

				T Desnos, V Orbovic, C Bellini, J Kronenberger, M Caboche, J Traas, and H Hofte Procuste1 mutants identify two distinct genetic pathways controlling hypocotyl cell elongation, respectively in dark- and light-grown Arabidopsis seedlings Development, January 2, 1996; 122(2): 683 - 693. [Abstract] [PDF]

				J. A. Ozga, R. van Huizen, and D. M. Reinecke Hormone and Seed-Specific Regulation of Pea Fruit Growth Plant Physiology, April 1, 2002; 128(4): 1379 - 1389. [Abstract] [Full Text] [PDF]