Plant Physiology 100:153-156 (1992)
© 1992 American Society of Plant Biologists
Environmental and Stress Physiology
Cytokinin Is Required to Induce the Nitrogen-Dependent Accumulation of mRNAs for Phosphoenolpyruvate Carboxylase and Carbonic Anhydrase in Detached Maize Leaves 1
Bambang Sugiharto2,
James N. Burnell and
Tatsuo Sugiyama
Department of Agricultural Chemistry, School of Agriculture, Nagoya University, Nagoya 464-01, Japan,
Centre for Molecular Biotechnology, Queensland University of Technology, PO Box 2434, Brisbane QLD 4001, Australia
Previous studies with intact maize (Zea mays L.) plants indicated that phosphoenolpyruvate carboxylase (PEPC) levels are controlled by nitrogen (N) availability and that this regulation is presumably at the transcriptional level (B. Sugiharto, K. Miyata, H. Nakamoto, H. Sasakawa, T. Sugiyama [1990] Plant Physiol 92: 963-969; B. Sugiharto, T. Sugiyama [1992] Plant Physiol 98: 1403-1408). In the present study, detached maize leaves were used to investigate further the mechanism of N-dependent regulation of gene expression in C4 plants. PEPC and carbonic anhydrase (CA) mRNA levels decreased in leaves detached from maize plants. Addition of high nitrate did not prevent this decrease. However, the addition of zeatin to solutions bathing the cut ends of the detached leaves inhibited the decrease of PEPC and CA mRNA levels. Simultaneous addition of high nitrate and zeatin to leaves detached from N-deficient maize plants caused a large and rapid increase in PEPC and CA mRNA levels. Zeatin could be replaced by benzyladenine, but not by indoleacetic acid or abscisic acid. Both CA isozymes were effected and responded in an identical manner. We conclude that detached maize leaves provide an excellent experimental system to study the mechanism(s) of N-mediated regulation of PEPC and CA gene expression. However, zeatin is an essential component of this system.
2 Present address: Faculty of Agriculture, Jember University, Kampus Tegalboto, Jember 68121, Indonesia.
1 This work was supported in part by grants-in-aid for Scientific Research on Priority Areas (No. 04273103) from the Ministry of Education, Science and Culture of Japan to T.S., by a Fellowship from the Japanese Society for the Promotion of Science to J.N.B., and by a Japanese Government Scholarship (The Ministry of Education, Science and Culture of Japan) to B.S.
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